| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPUSD3 |
| Uniprot No | Q6P087 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351 aa |
| 活性数据 | MRAVLAREMD GRRVLGRFWS GWRRGLGVRP VPEDAGFGTE ARHQRQPRGS CQRSGPLGDQ PFAGLLPKNL SREELVDALR AAVVDRKGPL VTLNKPQGLP VTGKPGELTL FSVLPELSQS LGLREQELQV VRASGKESSG LVLLSSCPQT ASRLQKYFTH ARRAQRPTAT YCAVTDGIPA ASEGKIQAAL KLEHIDGVNL TVPVKAPSRK DILEGVKKTL SHFRVVATGS GCALVQLQPL TVFSSQLQVH MVLQLCPVLG DHMYSARVGT VLGQRFLLPA ENNKPQRQVL DEALLRRLHL TPSQAAQLPL HLHLHRLLLP GTRARDTPVE LLAPLPPYFS RTLQCLGLRL Q |
| 分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RPUSD3蛋白的假设性参考文献示例(实际文献请通过学术数据库核实)：
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**1. 文献名称**: *RPUSD3 modulates RNA pseudouridylation and promotes hepatocellular carcinoma progression*
**作者**: Li, X., et al.
**摘要**: 研究揭示了RPUSD3通过催化特定mRNA的假尿苷修饰，激活Wnt/β-catenin信号通路，从而促进肝癌细胞增殖和转移，为肝癌治疗提供了潜在靶点。
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**2. 文献名称**: *Structural insights into the catalytic mechanism of human RPUSD3 pseudouridine synthase*
**作者**: Wang, Y., et al.
**摘要**: 通过X射线晶体学解析了RPUSD3蛋白的三维结构，阐明了其催化假尿苷合成的关键活性位点，为设计小分子抑制剂奠定结构基础。
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**3. 文献名称**: *RPUSD3 interacts with mitochondrial RNA and regulates oxidative phosphorylation*
**作者**: Garcia, M., et al.
**摘要**: 发现RPUSD3在线粒体RNA假尿苷化中起关键作用，其缺失导致呼吸链复合物功能受损，提示其在能量代谢疾病中的潜在作用。
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**注**：以上内容为模拟示例，实际文献需在PubMed、Web of Science等平台以“RPUSD3”、“recombinant RPUSD3”等关键词检索。
**Recombinant Human RPUSD3 Protein: Background and Significance**
Recombinant human RPUSD3 (RNA Pseudouridine Synthase Domain-Containing 3) protein is a genetically engineered version of the endogenous enzyme involved in RNA modification. RPUSD3 belongs to the pseudouridine synthase family, which catalyzes the isomerization of uridine to pseudouridine (Ψ), a common RNA modification critical for stabilizing RNA structure, fine-tuning translation, and regulating cellular processes. This protein contains a conserved catalytic domain typical of pseudouridine synthases, suggesting its role in site-specific Ψ formation in ribosomal RNA (rRNA) or other RNA molecules.
RPUSD3 is expressed in various tissues and implicated in ribosome biogenesis, cellular stress responses, and possibly cancer progression. Studies highlight its interaction with RNA-guided machinery or other modifiers, though its precise substrates and mechanistic details remain under investigation. Recombinant RPUSD3 is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and structural studies. Its purified form facilitates research on RNA modification pathways, enzyme kinetics, and interactions with RNA targets or partners.
As dysregulated RNA modifications are linked to diseases like cancer and neurodegeneration, recombinant RPUSD3 serves as a tool to explore therapeutic targeting of pseudouridylation pathways. Ongoing research aims to unravel its physiological roles, substrate specificity, and potential as a biomarker or drug target.
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