| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRN3 |
| Uniprot No | Q9NYV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-106 aa |
| 活性数据 | MRALENDFFNSPPRKTVRFGGTVTEVLLKYKKGETNDFELLKNQLLDPDIKDDQIINWLLEFRSSVMYLTKDFEQLISIILRLPWLNRSQTVVEEYLAFLGNLVSA |
| 分子量 | 38.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RRN3蛋白的3篇参考文献示例，包括文献名称、作者及摘要概要：
1. **文献名称**：**"RRN3 is essential for ribosomal RNA transcription in vivo and interacts with RNA polymerase I"**
**作者**：Bodemeyer et al. (2000)
**摘要**：研究发现RRN3是哺乳动物细胞中RNA聚合酶I介导的rRNA转录的必需因子。通过基因敲除实验，证实RRN3缺失导致rRNA合成终止;重组人RRN3蛋白的导入可恢复转录活性，表明其在转录起始复合物组装中的核心作用。
2. **文献名称**：**"Recombinant human TIF-IA/RRN3 enhances ribosomal DNA transcription in vitro"**
**作者**：Yuan et al. (2005)
**摘要**：报道了在大肠杆菌系统中高效表达和纯化重组人RRN3蛋白的方法，并在体外转录实验中证明其能够结合RNA聚合酶I，显著增强rDNA模板的转录效率，为研究核糖体生物发生提供了工具。
3. **文献名称**：**"Phosphorylation-dependent regulation of RRN3 activity in cell cycle progression"**
**作者**：Wu et al. (2012)
**摘要**：揭示RRN3的磷酸化修饰调控其在细胞周期中的功能。通过重组突变体蛋白实验，发现特定位点的磷酸化影响RRN3与RNA聚合酶I的结合能力，从而协调rRNA合成与细胞增殖进程。
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注：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“RRN3”、“TIF-IA”、“recombinant”检索最新研究。
**Background of Recombinant Human RRN3 Protein**
Recombinant human RRN3 (RRN3 homolog, RNA polymerase I transcription factor), also known as TIF-IA, is a critical regulatory protein involved in RNA polymerase I (Pol I)-mediated transcription of ribosomal RNA (rRNA). RRN3 acts as a bridging factor that links Pol I to the ribosomal DNA (rDNA) promoter, facilitating the assembly of the transcription initiation complex. Its activity is essential for the production of rRNA, a fundamental step in ribosome biogenesis, which directly impacts cellular growth, proliferation, and homeostasis.
Structurally, RRN3 contains conserved domains that mediate interactions with Pol I, the rDNA promoter-associated factor UBTF/UBF, and other regulatory components like the selectivity factor SL1. Its function is tightly regulated by post-translational modifications, including phosphorylation, which modulates its binding affinity and recruitment to rDNA loci in response to cellular signals such as nutrient availability, stress, or growth factor stimulation. Dysregulation of RRN3 has been implicated in diseases characterized by uncontrolled cell proliferation, including cancers, making it a potential therapeutic target.
Recombinant human RRN3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains native biochemical properties. It is widely used *in vitro* to study Pol I transcription mechanisms, screen inhibitors, or analyze mutations affecting rRNA synthesis. Research on RRN3 continues to unravel its role in cellular adaptation and disease, offering insights into ribosomopathies and cancer therapeutics.
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