| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTF1 |
| Uniprot No | Q92541 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-710 aa |
| 活性数据 | MRGRLCVGRA AAAAAAVAVP LAGGQEGSPG GGRRGSRGTT MVKKRKGRVV IDSDTEDSGS DENLDQELLS LAKRKRSDSE EKEPPVSQPA ASSDSETSDS DDEWTFGSNK NKKKGKARKI EKKGTMKKQA NKTASSGSSD KDSSAESSAP EEGEVSDSDS NSSSSSSDSD SSSEDEEFHD GYGEDLMGDE EDRARLEQMT EKEREQELFN RIEKREVLKR RFEIKKKLKT AKKKEKKEKK KKQEEEQEKK KLTQIQESQV TSHNKERRSK RDEKLDKKSQ AMEELKAERE KRKNRTAELL AKKQPLKTSE VYSDDEEEEE DDKSSEKSDR SSRTSSSDEE EEKEEIPPKS QPVSLPEELN RVRLSRHKLE RWCHMPFFAK TVTGCFVRIG IGNHNSKPVY RVAEITGVVE TAKVYQLGGT RTNKGLQLRH GNDQRVFRLE FVSNQEFTES EFMKWKEAMF SAGMQLPTLD EINKKELSIK EALNYKFNDQ DIEEIVKEKE RFRKAPPNYA MKKTQLLKEK AMAEDLGDQD KAKQIQDQLN ELEERAEALD RQRTKNISAI SYINQRNREW NIVESEKALV AESHNMKNQQ MDPFTRRQCK PTIVSNSRDP AVQAAILAQL NAKYGSGVLP DAPKEMSKGQ GKDKDLNSKS ASDLSEDLFK VHDFDVKIDL QVPSSESKAL AITSKAPPAK DGAPRRSLNL EDYKKRRGLI |
| 分子量 | 80.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RTF1蛋白的3条参考文献的概括(文献为假设性示例，仅作参考)：
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1. **文献名称**：*Rtf1 Couples Histone H3K36 Methylation to Transcription Elongation*
**作者**：Miller TC et al.
**摘要**：研究揭示了人RTF1蛋白作为Paf1复合体亚基，在RNA聚合酶II介导的转录延伸中起关键作用，通过促进组蛋白H3K36的三甲基化(H3K36me3)调控染色质修饰和基因表达。实验表明RTF1缺失会导致转录延伸障碍及H3K36me3水平下降。
2. **文献名称**：*Rtf1-Dependent H2B Ubiquitylation Regulates Transcriptional Termination and mRNA Splicing*
**作者**：Zhang Y et al.
**摘要**：本文证明RTF1通过介导组蛋白H2B的单泛素化(H2Bub1)影响转录终止和pre-mRNA剪接。研究结合ChIP-seq分析，显示RTF1的缺失导致异常的转录延伸停滞及剪接效率降低，提示其在表观遗传调控中的多效性功能。
3. **文献名称**：*Oncogenic Role of RTF1 in Colorectal Cancer via c-Myc Activation*
**作者**：Lee S et al.
**摘要**：该研究报道RTF1在结直肠癌中异常高表达，并通过增强c-Myc原癌基因的转录促进肿瘤细胞增殖。靶向RTF1的siRNA实验显示其敲低显著抑制肿瘤生长，提示RTF1作为潜在治疗靶点。
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*注：以上文献为假设示例，实际研究中可检索PubMed等数据库获取真实文献(如搜索“Human RTF1”或“PAF1 complex”)。*
Recombinant human RTF1 protein is a genetically engineered form of the endogenous Rtf1 (RNA polymerase II-associated transcription factor 1), a conserved eukaryotic protein involved in transcriptional regulation and chromatin remodeling. Rtf1 is a key component of the Paf1 complex (PAF1C), which associates with RNA polymerase II during transcription elongation. It plays critical roles in histone modification, including promoting histone H2B ubiquitination and H3K4 methylation, processes essential for gene activation and epigenetic regulation. Studies link Rtf1 to developmental processes, cellular differentiation, and DNA damage response, with dysregulation implicated in cancers and neurological disorders.
Recombinant RTF1 is typically produced using expression systems like *E. coli* or mammalian cells, followed by affinity purification to ensure high purity and activity. Its recombinant form enables detailed biochemical studies, such as elucidating structural domains (e.g., the Plus3 domain for interaction with phosphorylated Pol II) or investigating mechanisms in transcription-coupled chromatin modification. Researchers also utilize it to model PAF1C-related diseases or screen therapeutic agents targeting transcriptional dysregulation. By providing a controlled, scalable source of functional protein, recombinant RTF1 accelerates both basic research and translational applications in epigenetics and molecular medicine.
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