| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUPT6H |
| Uniprot No | Q7KZ85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUPT6H重组蛋白的3篇参考文献及其摘要概括：
1. **"SUPT6H is a checkpoint of the DNA replication fork network that regulates genome stability"**
*作者：Bianchi et al.*
摘要：该研究通过重组SUPT6H蛋白的体外实验，揭示了其在DNA复制叉稳定性中的作用，证明其通过调控染色质重塑维持基因组完整性。
2. **"Structural and functional analysis of the Spt6 core domain and its role in transcription elongation"**
*作者：Winkler et al.*
摘要：利用重组SUPT6H蛋白的晶体结构解析，阐明其核心结构域如何与RNA聚合酶II及核小体相互作用，促进转录延伸过程中染色质的再组装。
3. **"SUPT6H coordinates histone H3K36 methylation and RNA polymerase II dynamics during transcription"**
*作者：Zhou et al.*
摘要：研究通过重组蛋白结合质谱分析，发现SUPT6H作为组蛋白伴侣，介导H3K36甲基化修饰，并协调RNA聚合酶II的转录进程。
4. **"FACT-mediated exchange of histone variant H2A.Z regulates transcriptional initiation and elongation"**
*作者：Jerzmanowski et al.*
摘要：文中利用重组SUPT6H与FACT复合体的共纯化实验，证明其协同调控H2A.Z组蛋白变体的替换，影响染色质可及性和转录活性。
*注：上述文献标题及作者为虚构示例，实际引用时需核实真实文献。*
SUPT6H (SUPT6H homolog, histone chaperone and transcription elongation factor) is a multifunctional nuclear protein involved in chromatin remodeling, transcriptional regulation, and epigenetic maintenance. It serves as a conserved component of the FACT (Facilitates Chromatin Transcription) complex, playing critical roles in DNA replication, RNA polymerase II-mediated transcription elongation, and histone recycling. Structurally, SUPT6H contains a conserved N-terminal SH2 domain that facilitates interactions with phosphorylated C-terminal domain (CTD) residues of RNA polymerase II, and a C-terminal acidic region for histone chaperone activity. This dual functionality enables SUPT6H to coordinate nucleosome reassembly during transcriptional processes, maintaining chromatin integrity while allowing transcriptional machinery progression.
Recombinant SUPT6H proteins are engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian expression systems with affinity tags (e.g., GST, His-tag) for purification. These recombinant versions retain key biochemical properties, enabling *in vitro* studies of chromatin dynamics, histone-DNA interactions, and transcription-coupled epigenetic modifications. Researchers utilize SUPT6H recombinant proteins to investigate its role in coordinating histone H3-H4 tetramer deposition, resolving transcription-replication conflicts, and facilitating DNA repair processes.
Emerging evidence links SUPT6H dysregulation to cancer progression, neurodevelopmental disorders, and viral pathogenesis, driving demand for functional recombinant forms. Current applications include chromatin immunoprecipitation (ChIP) workflows, nucleosome reconstitution assays, and high-throughput screening for therapeutic agents targeting transcription-coupled chromatin remodeling. The recombinant protein's stability and preserved functional domains make it particularly valuable for mechanistic studies of epigenetic inheritance and transcriptional fidelity.
×
