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Recombinant Human TWIST protein

  • 中文名: Twist转录因子(TWIST)重组蛋白
  • 别    名: TWIST;BHLHA38;TWIST;Twist-related protein 1
货号: PA1000-7962
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点TWIST
Uniprot NoQ15672
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-202aa
氨基酸序列MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGA GPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSP QSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLK LAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSA SH
预测分子量48 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TWIST重组蛋白的3篇参考文献,按研究主题分类列举:

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### 1. **"Expression and purification of recombinant TWIST1 protein for structural studies"**

**作者**: Zhang Y, et al.

**摘要**: 该研究报道了人源TWIST1转录因子的重组表达与纯化方法,利用大肠杆菌系统优化了可溶性蛋白产量,并通过圆二色光谱验证其螺旋-环-螺旋结构域的正确折叠,为后续结构功能研究奠定基础。

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### 2. **"Functional analysis of TWIST recombinant protein in epithelial-mesenchymal transition (EMT)"**

**作者**: Lee JM, et al.

**摘要**: 通过哺乳动物细胞表达系统获得重组TWIST蛋白,证明其可诱导乳腺癌细胞发生EMT,激活下游靶基因(如N-cadherin),并显著增强细胞迁移能力,揭示了TWIST在癌症转移中的分子机制。

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### 3. **"Crystal structure of the TWIST-bHLH domain bound to E-box DNA"**

**作者**: Chen Z, et al.

**摘要**: 首次解析了重组TWIST蛋白的bHLH结构域与DNA结合复合物的晶体结构,揭示了其特异性识别E-box序列(5'-CANNTG-3')的关键氨基酸残基,为设计靶向TWIST的小分子抑制剂提供结构依据。

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**说明**:上述文献标题与内容为示例性概括,实际引用时需以具体发表的论文为准。建议通过PubMed或Web of Science以关键词“TWIST recombinant protein”搜索最新研究。

背景信息

**Background of TWIST Recombinant Proteins**

TWIST proteins, belonging to the basic helix-loop-helix (bHLH) transcription factor family, play pivotal roles in embryonic development, cell differentiation, and tissue homeostasis. The TWIST family includes TWIST1 and TWIST2. which regulate processes such as mesoderm formation, craniofacial development, and limb patterning by modulating gene expression through DNA binding and protein-protein interactions. Structurally, TWIST proteins contain a conserved bHLH domain enabling dimerization with other bHLH partners (e.g., E-proteins) to bind E-box motifs in target gene promoters.

In pathological contexts, TWIST1 is widely implicated in cancer progression, particularly in epithelial-mesenchymal transition (EMT), metastasis, and chemoresistance. By repressing epithelial markers (e.g., E-cadherin) and activating mesenchymal genes, TWIST1 enhances tumor cell invasiveness and survival. Its overexpression correlates with poor prognosis in multiple cancers, including breast, prostate, and gastric carcinomas. TWIST2 shares functional redundancy with TWIST1 but exhibits tissue-specific roles, such as regulating adipogenesis and immune responses.

Recombinant TWIST proteins are engineered to study these mechanisms *in vitro* or *in vivo*. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, recombinant TWIST retains DNA-binding activity and interaction capabilities, serving as a tool for elucidating signaling pathways (e.g., TGF-β, Wnt) or screening inhibitors. Tagged versions (e.g., His, GST) facilitate purification and detection in assays.

Additionally, TWIST recombinant proteins hold therapeutic potential. For instance, targeting TWIST-mediated EMT may suppress metastasis, while modulating TWIST activity could aid regenerative medicine by influencing stem cell differentiation. However, challenges remain in achieving tissue-specific delivery and minimizing off-target effects.

Overall, TWIST recombinant proteins are critical for unraveling developmental and disease-related processes, offering avenues for novel diagnostics and therapies.

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