Recombinant Human NOS1 protein

中文名： 神经型一氧化氮合酶(NOS1)重组蛋白
别名： NOS1；Nitric oxide synthase 1

货号: PA1000-9283

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	NOS1
Uniprot No	P29475
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-1434aa
氨基酸序列	MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS
预测分子量	160 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于NOS1重组蛋白的3篇参考文献示例(注：文献为模拟示例，非真实存在)：

1. **《高效表达与纯化重组大鼠NOS1的结构域》**

- 作者：A. Brown, C. Davis

- 摘要：通过原核表达系统成功表达了大鼠NOS1的氧酶结构域，优化纯化流程并获得高纯度蛋白，用于后续晶体结构解析。

2. **《重组人NOS1磷酸化修饰对其酶活性的调控研究》**

- 作者：M. Zhang 等

- 摘要：利用哺乳动物细胞表达系统制备重组人NOS1.证实特定位点磷酸化可通过影响钙调蛋白结合抑制一氧化氮合成活性。

3. **《昆虫细胞中重组NOS1/复合体的制备及功能分析》**

- 作者：T. Müller 等

- 摘要：采用杆状病毒系统共表达人NOS1与伴侣蛋白，获得具有完整酶活性的复合体，揭示辅助因子对蛋白稳定性的关键作用。

4. **《定向突变对重组NOS1电子传递效率的影响》**

- 作者：K. Sato 等

- 摘要：通过定点突变技术探究NOS1还原酶结构域中关键氨基酸对电子转移路径的调控，为酶工程改造提供依据。

(注：以上文献为虚构示例，实际研究中建议通过PubMed或SciFinder以“NOS1 recombinant protein”为关键词检索最新论文。)

背景信息

**Background of NOS1 Recombinant Protein**

Nitric oxide synthase 1 (NOS1), also known as neuronal NOS (nNOS), is a critical enzyme responsible for synthesizing nitric oxide (NO), a versatile signaling molecule involved in numerous physiological processes. NOS1 is predominantly expressed in neuronal tissues but is also found in skeletal muscle, epithelial cells, and other non-neuronal tissues. It plays a key role in neurotransmission, vascular regulation, immune response, and synaptic plasticity by catalyzing the conversion of L-arginine to L-citrulline and NO in a calcium/calmodulin-dependent manner. Dysregulation of NOS1 activity is linked to neurological disorders (e.g., Alzheimer’s disease, schizophrenia), cardiovascular diseases, and cancer.

Recombinant NOS1 protein is engineered through heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to study its structure, function, and interactions. The recombinant form typically includes affinity tags (e.g., His-tag) for purification and may retain functional domains, such as the oxygenase and reductase regions, as well as regulatory motifs like the PDZ domain for subcellular targeting. Researchers utilize NOS1 recombinant protein to investigate enzyme kinetics, post-translational modifications (e.g., phosphorylation), and its role in NO-mediated signaling pathways. It also serves as a tool for drug discovery, enabling high-throughput screening of inhibitors or activators to modulate pathological NO overproduction or deficiency. Studies leveraging recombinant NOS1 have advanced understanding of its dimerization mechanism, redox sensitivity, and interactions with scaffolding proteins (e.g., PSD-95), offering insights into therapeutic strategies for NOS1-associated diseases.