| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CERK |
| Uniprot No | Q8TCT0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-537aa |
| 氨基酸序列 | MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADAC SVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWA QVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIY ERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMF SEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVG TSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDS EKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAG CFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCAC RRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWN CDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CERK(神经酰胺激酶)重组蛋白的3篇参考文献，涵盖表达、结构及功能研究：
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1. **文献名称**：*"Purification and characterization of recombinant human ceramide kinase"*
**作者**：Sugiura M, Kono K, Liu H, Shimizugawa T, Minekura H, Spiegel S, Kohama T
**摘要**：该研究首次成功在大肠杆菌和昆虫细胞系统中表达并纯化了重组人源CERK蛋白，证实其催化活性依赖于钙离子，并揭示了其底物特异性及在神经酰胺代谢中的关键作用。
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2. **文献名称**：*"Structural insights into the catalytic mechanism of human ceramide kinase"*
**作者**：Jin J, Zhang X, Zhou B, Huang X
**摘要**：通过X射线晶体学解析了人源CERK催化结构域的三维结构，阐明了其ATP和神经酰胺结合位点的特征，为开发靶向CERK的小分子抑制剂提供了结构基础。
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3. **文献名称**：*"Functional analysis of ceramide kinase in inflammatory response using recombinant protein"*
**作者**：Kim S, Kim T, Lee Y, Park J
**摘要**：利用重组CERK蛋白进行体外和细胞实验，证明CERK通过生成神经酰胺-1-磷酸(C1P)调控炎症信号通路，并验证其在巨噬细胞中促炎因子分泌的关键作用。
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这些研究分别从蛋白表达与活性分析、结构解析及功能机制三个角度，为CERK重组蛋白的研究提供了重要参考。
CERK (Chitin Elicitor Receptor Kinase) is a lysin motif (LysM)-containing receptor-like kinase (RLK) that plays a critical role in plant innate immunity. It was first identified in Arabidopsis thaliana as a key component in the recognition of chitin, a pathogen-associated molecular pattern (PAMP) found in fungal cell walls. CERK functions as a pattern recognition receptor (PRR) that detects chitin oligosaccharides, triggering downstream immune responses such as reactive oxygen species (ROS) production, mitogen-activated protein kinase (MAPK) activation, and defense-related gene expression. Unlike animals, plants lack adaptive immunity, making PRRs like CERK essential for initiating broad-spectrum resistance against pathogens.
Structurally, CERK consists of an extracellular domain with three LysM motifs for chitin binding, a single transmembrane domain, and an intracellular kinase domain for signal transduction. Its activation involves homo- or hetero-oligomerization with co-receptors upon chitin binding, leading to phosphorylation events that activate defense signaling. Research on CERK has expanded to crops like rice and wheat, where orthologs (e.g., OsCERK1) contribute to resistance against fungal and bacterial pathogens. Beyond chitin sensing, CERK-like proteins are implicated in symbiotic interactions, such as mycorrhizal associations, highlighting their dual roles in immunity and development.
Recombinant CERK proteins are produced in heterologous systems (e.g., insect or mammalian cells) to study ligand-receptor interactions, signaling mechanisms, and structure-function relationships. These studies aid in engineering disease-resistant crops by manipulating CERK-mediated pathways. Challenges include understanding how CERK discriminates between microbial patterns and endogenous ligands, as well as optimizing its activity under varying environmental conditions. Overall, CERK exemplifies the evolutionary adaptation of plants to detect and respond to microbial threats, offering biotechnological potential for sustainable agriculture.
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