| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPTX2 |
| Uniprot No | P47972 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 氨基酸序列 | MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQG GAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGG KARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVS NAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTES TLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAF TICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQL PLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVL ILGQEQDTVGGRFDATQAFVGELSQFNIWDRVLRAQEIVNIANCSTNMPG NIIPWVDNNVDVFGGASKWPVETCEERLLDL |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPTX2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA receptor–YAP axis*
**作者**：Yang C, et al.
**摘要**：该研究揭示了NPTX2重组蛋白通过结合AMPA受体并激活下游YAP信号通路，促进肾透明细胞癌的增殖和转移，为癌症治疗提供了潜在靶点。
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2. **文献名称**：*Recombinant NPTX2 protein enhances synaptic plasticity and restores cognitive deficits in Alzheimer’s disease models*
**作者**：Xia M, et al.
**摘要**：研究者利用重组NPTX2蛋白干预阿尔茨海默病小鼠模型，发现其通过调控突触蛋白表达改善突触可塑性和认知功能，提示其在神经退行性疾病中的治疗潜力。
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3. **文献名称**：*Structural characterization of human neuronal pentraxin 2 and its interaction with amyloid-β*
**作者**：Lee SJ, et al.
**摘要**：通过重组表达纯化人源NPTX2蛋白，解析其晶体结构，并发现其与β-淀粉样蛋白(Aβ)的相互作用可能参与阿尔茨海默病的病理进程。
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注：以上文献为示例，实际引用时需根据具体研究内容核对真实文献信息。
**Background of NPTX2 Recombinant Protein**
Neuronal pentraxin 2 (NPTX2), also known as neuronal activity-regulated pentraxin, is a member of the pentraxin family of proteins traditionally associated with immune regulation. Unlike its homologs (e.g., C-reactive protein), NPTX2 is predominantly expressed in the central nervous system (CNS), where it plays a critical role in synaptic plasticity, neural circuit assembly, and long-term strengthening of excitatory synapses. It interacts with AMPA-type glutamate receptors, facilitating their clustering at postsynaptic membranes, thereby modulating synaptic transmission and plasticity.
NPTX2 is synthesized as a secreted glycoprotein and forms stable oligomers via its N-terminal domain, while its C-terminal pentraxin domain mediates ligand binding. Dysregulation of NPTX2 has been implicated in neurological and psychiatric disorders, including Alzheimer’s disease, schizophrenia, and autism spectrum disorders. Reduced NPTX2 levels correlate with synaptic loss and cognitive decline, highlighting its importance in maintaining neural connectivity.
Recombinant NPTX2 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce functional, purified protein for research and therapeutic development. It retains the ability to bind neuronal receptors and modulate synaptic activity, making it a valuable tool for studying synaptic mechanisms, drug screening, and exploring therapeutic strategies for CNS disorders. Its recombinant form is also investigated for potential diagnostic applications, as altered NPTX2 expression in cerebrospinal fluid or blood may serve as a biomarker for neurodegenerative conditions. Ongoing research aims to elucidate its precise molecular interactions and therapeutic potential in restoring synaptic function.
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