| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAV1 |
| Uniprot No | Q9H4B6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-383 aa |
| 活性数据 | MLSRKKTKNE VSKPAEVQGK YVKKETSPLL RNLMPSFIRH GPTIPRRTDI CLPDSSPNAF STSGDVVSRN QSFLRTPIQR TPHEIMRRES NRLSAPSYLA RSLADVPREY GSSQSFVTEV SFAVENGDSG SRYYYSDNFF DGQRKRPLGD RAHEDYRYYE YNHDLFQRMP QNQGRHASGI GRVAATSLGN LTNHGSEDLP LPPGWSVDWT MRGRKYYIDH NTNTTHWSHP LEREGLPPGW ERVESSEFGT YYVDHTNKKA QYRHPCAPSV PRYDQPPPVT YQPQQTERNQ SLLVPANPYH TAEIPDWLQV YARAPVKYDH ILKWELFQLA DLDTYQGMLK LLFMKELEQI VKMYEAYRQA LLTELENRKQ RQQWYAQQHG KNF |
| 分子量 | 44.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SAAV1(SAV1)蛋白相关的文献摘要示例，仅供参考(需根据实际文献修正)：
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1. **Title**: "Recombinant Expression and Functional Characterization of Human SAV1 in the Hippo Signaling Pathway"
**Authors**: Zhang, L., et al.
**Summary**: 研究通过大肠杆菌系统重组表达人SAV1蛋白，分析其与MST1/2激酶的相互作用，证实SAV1作为支架蛋白对Hippo通路下游效应因子YAP的磷酸化调控作用，为体外研究通路机制提供工具。
2. **Title**: "Structural Insights into SAV1’s Role in Tumor Suppression via Hippo Pathway Regulation"
**Authors**: Wang, Q., et al.
**摘要**: 通过X射线晶体学解析SAV1的结构，揭示其WW结构域和SARAH结构域的构象，阐明SAV1与MST1/2形成复合物的分子机制，并探讨其在癌症中突变导致通路失调的分子基础。
3. **Title**: "SAV1 Knockdown Enhances Cancer Cell Proliferation via YAP Activation: Implications for Therapeutic Targeting"
**Authors**: Kim, T., et al.
**摘要**: 利用重组SAV1蛋白体外验证其在肝癌细胞中的抑癌功能，实验表明SAV1缺失导致YAP核转位加速，促进肿瘤生长，提示恢复SAV1表达可能作为潜在治疗策略。
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**提示**：具体文献需通过学术数据库(如PubMed、Web of Science)检索关键词“recombinant human SAV1”或“SAV1 Hippo pathway”获取，建议结合近五年研究以反映前沿进展。
The Salvador homolog 1 (SAV1) protein is a critical component of the Hippo signaling pathway, an evolutionarily conserved network that regulates organ size, cell proliferation, apoptosis, and stem cell self-renewal. SAV1. encoded by the *SAV1* gene in humans, acts as a scaffolding protein to facilitate interactions between upstream kinases (MST1/2) and downstream effectors (LATS1/2) within the pathway. Structurally, SAV1 contains WW domains and a coiled-coil region, enabling protein-protein interactions essential for Hippo pathway activation. Upon activation, the Hippo pathway phosphorylates and inhibits YAP/TAZ transcription co-activators, thereby suppressing pro-growth and anti-apoptotic gene expression.
Recombinant human SAV1 protein is produced through genetic engineering in heterologous systems (e.g., *E. coli* or mammalian cells) to study its biochemical functions and therapeutic potential. Research highlights its role in tumor suppression, as SAV1 dysfunction or downregulation is linked to cancers, including liver, lung, and colorectal malignancies. Additionally, SAV1 influences tissue regeneration and immune responses, making it a target for regenerative medicine and cancer therapy. Recombinant SAV1 enables in vitro studies of Hippo pathway dynamics, kinase assays, and drug screening. Its application extends to understanding developmental processes, cellular homeostasis, and pathological mechanisms, underscoring its value in both basic and translational biomedical research.
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