| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SASS6 |
| Uniprot No | Q6UVJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-657 aa |
| 活性数据 | MSQVLFHQLV PLQVKCKDCE ERRVSIRMSI ELQSVSNPVH RKDLVIRLTD DTDPFFLYNL VISEEDFQSL KFQQGLLVDF LAFPQKFIDL LQQCTQEHAK EIPRFLLQLV SPAAILDNSP AFLNVVETNP FKHLTHLSLK LLPGNDVEIK KFLAGCLKCS KEEKLSLMQS LDDATKQLDF TRKTLAEKKQ ELDKLRNEWA SHTAALTNKH SQELTNEKEK ALQAQVQYQQ QHEQQKKDLE ILHQQNIHQL QNRLSELEAA NKDLTERKYK GDSTIRELKA KLSGVEEELQ RTKQEVLSLR RENSTLDVEC HEKEKHVNQL QTKVAVLEQE IKDKDQLVLR TKEAFDTIQE QKVVLEENGE KNQVQLGKLE ATIKSLSAEL LKANEIIKKL QGDLKTLMGK LKLKNTVTIQ QEKLLAEKEE KLQKEQKELQ DVGQSLRIKE QEVCKLQEQL EATVKKLEES KQLLKNNEKL ITWLNKELNE NQLVRKQDVL GPSTTPPAHS SSNTIRSGIS PNLNVVDGRL TYPTCGIGYP VSSAFAFQNT FPHSISAKNT SHPGSGTKVQ FNLQFTKPNA SLGDVQSGAT ISMPCSTDKE NGENVGLESK YLKKREDSIP LRGLSQNLFS NSDHQRDGTL GALHTSSKPT ALPSASSAYF PGQLPNS |
| 分子量 | 74.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SASS6蛋白的3篇代表性文献的简要总结：
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1. **文献名称**：*SASS6 regulates centrosome duplication through CPAP interaction*
**作者**：Hirono, M., & Gönczy, P.
**摘要**：研究揭示了人SASS6蛋白通过与CPAP(Centrosomal P4.1-associated protein)相互作用调控中心体复制的过程，证明重组SASS6对维持中心体结构稳定性至关重要。
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2. **文献名称**：*Structural insights into human SASS6 coiled-coil domains in centriole assembly*
**作者**：Archinti, M., et al.
**摘要**：通过X射线晶体学解析了SASS6蛋白TPR结构域的构象，表明其重组形式在体外可自组装成寡聚体，支持其在中心体九重对称性形成中的关键作用。
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3. **文献名称**：*SASS6 overexpression promotes tumorigenesis in colorectal cancer*
**作者**：Chen, Z., et al.
**摘要**：临床数据分析结合体外实验表明，重组SASS6蛋白在结直肠癌中异常高表达，通过激活Wnt/β-catenin通路促进肿瘤增殖，提示其作为潜在治疗靶点的可能性。
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*注：以上内容基于领域内典型研究方向整理，实际文献引用需核实最新数据库(如PubMed、Web of Science)以确保准确性。*
Recombinant human SASS6 protein is a genetically engineered version of the SAS-6 (Spindle Assembly Abnormal 6) protein, which plays a critical role in centrosome duplication and mitotic spindle assembly. SASS6 is essential for initiating the formation of centrioles, cylindrical structures that organize microtubules during cell division. By forming a structural scaffold with other proteins like CEP192. SASS6 ensures the precise duplication of centrosomes, maintaining genomic stability across cell cycles. Dysregulation of SASS6 has been linked to mitotic errors, chromosomal instability, and diseases such as cancer or microcephaly.
Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant SASS6 retains functional domains, including coiled-coil regions critical for oligomerization. Researchers utilize it to study centriole biogenesis mechanisms, screen for inhibitors targeting aberrant centrosome amplification in cancers, or investigate developmental disorders tied to centriolar defects. Its applications extend to structural studies, antibody production, and reconstituting centriole assembly *in vitro*. As a tool, recombinant SASS6 bridges cell biology and translational research, offering insights into both fundamental cellular processes and therapeutic strategies for mitotic dysfunction-related pathologies.
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