| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCAND2 |
| Uniprot No | Q9GZW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306 aa |
| 活性数据 | MAVAVDQQIQ TPSVQDLQIV KLEEDSHWEQ EISLQGNYPG PETSCQSFWH FRYQEASRPR EALLQLQKLC CQWLRPEKCT KEQILELLVL EQFPTVLLQE IQIWVRQQHP ESGEEAVALV EDLQKEPGRQ RLEPRARPSG RTPPAQLRSP WPMTAAGPAS RARASETGST ASCAGRWRTC CAAAAAPSAA RSASARTGRS TSSCARAARA PSATEGALTR TPAPRRPLQR RRPGTGPWRP GRQRGAGTAP PGTQPRQRPS PRPTPRRPRP RLARPRAGQK PWLLVIRSME RVMYVMLIIQ METEDV |
| 分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人源SCAND2蛋白研究的参考文献概述(注：以下为模拟示例，具体文献信息需根据实际数据库检索确认)：
1. **标题**: "SCAND2 regulates chromatin accessibility and transcriptional repression in human cells"
**作者**: Smith J, et al.
**摘要**: 研究揭示了SCAND2蛋白通过结合染色质修饰复合物(如Polycomb group)调控基因沉默的机制。利用重组人SCAND2蛋白进行体外互作实验，证实其SCAN结构域介导了与特定转录因子的结合，可能参与肿瘤抑制通路。
2. **标题**: "Structural and functional analysis of the SCAN domain of SCAND2"
**作者**: Kim S, et al.
**摘要**: 通过重组表达并纯化人SCAND2蛋白的SCAN结构域，采用X射线晶体学解析其三维结构，发现其通过二聚化形式与其他核蛋白(如ZNF202)结合，调控下游靶基因的表达。
3. **标题**: "SCAND2 expression modulates cellular differentiation in embryonic stem cells"
**作者**: García-Pérez R, et al.
**摘要**: 利用重组SCAND2蛋白处理小鼠胚胎干细胞，发现其通过抑制Oct4启动子活性促进分化进程，提示SCAND2在干细胞多能性调控中的潜在作用。
提示：实际文献检索建议通过PubMed/Google Scholar使用关键词“SCAND2”、“recombinant SCAND2 protein”或“SCAN domain protein 2”进行查询，并重点关注2015年后发表于《Nucleic Acids Research》《Biochim Biophys Acta》等期刊的研究。
SCAND2 (SCAN domain-containing protein 2) is a member of the SCAN domain family, characterized by a conserved ~100-amino acid SCAN domain implicated in protein-protein interactions and transcriptional regulation. This nuclear protein is closely associated with the KRAB-ZFP (Krüppel-associated box zinc finger protein) family, which plays roles in chromatin remodeling and gene silencing. The SCAN domain enables SCAND2 to dimerize or interact with other SCAN-containing proteins, forming regulatory complexes that influence gene expression by recruiting epigenetic modifiers.
Functionally, SCAND2 is suggested to act as a transcriptional co-repressor, modulating the activity of zinc finger transcription factors. It may compete with other SCAN domain proteins for binding partners, fine-tuning transcriptional networks involved in development, differentiation, and cellular homeostasis. Studies link SCAND2 to chromatin organization and the repression of retrotransposons, contributing to genome stability.
Recombinant SCAND2 protein is typically produced in bacterial or mammalian expression systems using genetic engineering techniques. Its purified form enables structural studies, interaction assays (e.g., co-immunoprecipitation, yeast two-hybrid), and functional analyses of its role in chromatin dynamics. Research on SCAND2 provides insights into epigenetic regulation, with potential implications in cancer (e.g., aberrant chromatin states) and neurological disorders. However, its precise mechanistic roles and disease associations remain under investigation, highlighting the need for further exploration of this regulatory protein.
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