| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SC5DL |
| Uniprot No | O75845 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299 aa |
| 活性数据 | MDLVLRVADYYFFTPYVYPATWPEDDIFRQAISLLIVTNVGAYILYFFCATLSYYFVFDHALMKHPQFLKNQVRREIKFTVQALPWISILTVALFLLEIRGYSKLHDDLGEFPYGLFELVVSIISFLFFTDMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYPFIFPLHKVVYLSLYILVNIWTISIHDGDFRVPQILQPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKNPSSFEGKGPLSYVKEMTEGKRSSHSGNGCKNEKLFNGEFTKTE |
| 分子量 | 61.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SC5DL蛋白的示例参考文献(虚构内容，供参考)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant Human SC5DL in Cholesterol Biosynthesis*
**作者**：Smith A, et al.
**摘要**：该研究通过在大肠杆菌系统中成功表达重组人SC5DL蛋白，验证其参与胆固醇合成途径的酶活性，证明其催化7-脱氢胆固醇转化为胆固醇的关键作用。
2. **文献名称**：*Structural Characterization of SC5DL Protein: Insights from X-ray Crystallography*
**作者**：Lee J, et al.
**摘要**：首次解析了重组SC5DL蛋白的晶体结构，揭示其底物结合位点及催化机制，为靶向胆固醇代谢疾病的药物设计提供结构基础。
3. **文献名称**：*SC5DL Deficiency and Its Association with Skin Disorders: A Study Using Recombinant Protein Models*
**作者**：Garcia R, et al.
**摘要**：通过构建重组SC5DL蛋白模型，发现其功能缺失与先天性角化不良等皮肤病的相关性，并提出可能的代谢补偿机制。
4. **文献名称**：*High-Yield Purification of Recombinant SC5DL for in vitro Enzyme Assays*
**作者**：Wang H, et al.
**摘要**：开发了一种高效纯化重组SC5DL蛋白的方法，并应用于高通量筛选抑制剂，为代谢综合征治疗提供实验工具。
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**注**：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。SC5DL蛋白主要涉及甾醇代谢，相关研究多聚焦于其在脂质代谢疾病或遗传病中的作用。
SC5DL (Sterol-C5-desaturase-like), also known as 7-dehydrocholesterol reductase (DHCR7), is a critical enzyme in the cholesterol biosynthesis pathway. It catalyzes the reduction of 7-dehydrocholesterol (7-DHC) to cholesterol, the final step in the Bloch pathway of cholesterol synthesis. This endoplasmic reticulum membrane-associated protein is encoded by the DHCR7 gene in humans. SC5DL plays a vital role in maintaining cellular cholesterol homeostasis and regulating precursor levels for vitamin D synthesis and steroid hormones.
Mutations in DHCR7 are linked to Smith-Lemli-Opitz syndrome (SLOS), an autosomal recessive disorder characterized by developmental abnormalities and impaired cholesterol production. Recombinant human SC5DL protein is produced using biotechnological systems like E. coli or mammalian cell cultures, enabling studies of its structure-function relationships, enzymatic mechanisms, and interactions with lipid substrates. Its recombinant form serves as a tool for drug screening, SLOS diagnostic assays, and investigating cholesterol-related signaling pathways, including Hedgehog protein maturation. Research on SC5DL also contributes to understanding the balance between cholesterol synthesis and dietary uptake, with implications for metabolic disease therapeutics. The protein's thermolabile nature and membrane-binding properties present unique challenges in purification, necessitating optimized expression systems for stability and activity retention.
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