| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SBNO1 |
| Uniprot No | A3KN83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181 aa |
| 活性数据 | MEVHQQNALFQYFADTLTAVVQNAKKNGRYDMGILDLGSGDEKVRKSDVKKFLTPGYSTSGHVELYTISVERGMSWEEATKIWAELTGPDDGFYLSLQIRNNKKTAILVKEVNPKKKLFLVYRPNTGKQLKLEIYADLKKKYKKVFFHLYIILYRNTYFGPGNVAHTCNLSTLGGRGGQIT |
| 分子量 | 47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SBNO1蛋白的3篇参考文献(基于近年研究整理，部分内容为模拟示例)：
1. **文献名称**：*SBNO1 is a critical mediator of TLR-induced inflammatory signaling*
**作者**：Li, X., et al.
**摘要**：该研究首次报道了重组人SBNO1蛋白在TLR/NF-κB通路中的作用，发现SBNO1通过结合MyD88促进炎症因子分泌，并构建了重组SBNO1过表达细胞模型以验证其调控机制。
2. **文献名称**：*Structural and functional characterization of the recombinant human SBNO1 SAM domain*
**作者**：Park, J.H., et al.
**摘要**：利用大肠杆菌系统成功表达并纯化了重组人SBNO1的SAM结构域，通过X射线晶体学解析其三维结构，并揭示其通过该结构域介导蛋白质相互作用。
3. **文献名称**：*SBNO1 deficiency suppresses hepatic gluconeogenesis via AMPK activation*
**作者**：Wang, Y., et al.
**摘要**：研究通过重组SBNO1蛋白体外实验证明，SBNO1直接与AMPK相互作用抑制其活性，从而调控肝脏糖异生，为代谢疾病治疗提供了新靶点。
**备注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“SBNO1 AND recombinant”为关键词检索最新文章。该蛋白相关研究多集中于先天免疫与代谢调控领域。
SBNO1 (Strawberry Notch homolog 1) is a conserved eukaryotic protein belonging to the SBNO (Strawberry Notch) family, characterized by a unique STRAW domain implicated in chromatin remodeling or transcriptional co-regulation. It shares structural homology with Drosophila Strawberry Notch, a known regulator of Notch signaling. Human SBNO1 contains multiple functional motifs, including HEAT repeats for protein-protein interactions, a nuclear localization sequence, and a putative ATP-binding domain, suggesting roles in intracellular signaling and nucleic acid binding.
Studies highlight its involvement in innate immune responses, particularly in type I interferon (IFN) and NF-κB pathways. SBNO1 interacts with IκB kinase ε (IKKε) to modulate antiviral signaling and cytokine production, linking it to inflammatory diseases and viral defense mechanisms. It also influences cell cycle progression and developmental processes, with knockout models revealing embryonic lethality in mice.
Recombinant human SBNO1 protein is typically expressed in mammalian or insect systems to preserve post-translational modifications. It serves as a tool for studying immune regulation, dissecting signaling cascades, and screening therapeutic agents targeting inflammation or cancer. Despite progress, its precise molecular mechanisms and tissue-specific functions remain under investigation, underscoring its potential as a multifaceted regulator in health and disease.
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