| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SBF2 |
| Uniprot No | Q86WG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100 aa |
| 活性数据 | MARLADYFIVVGYDHEKPGSGEGLGKIIQRFPQKDWDDTPFPQGIELFCQPGGWQLSRERKQPTFFVVVLTDIDSDRHYCSCLTFYEAEINLQGTKKEEI |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与“重组人SBF2蛋白”相关的假设性参考文献(注：因实际文献有限，以下内容基于相关领域常见研究方向模拟编写)：
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1. **文献名称**：*Functional Characterization of Recombinant Human SBF2 Protein in Myelination Pathways*
**作者**：A. Müller et al.
**摘要**：研究通过大肠杆菌系统表达了重组人SBF2蛋白，证实其与肌管素磷酸酶(MTMR13)相互作用，调节周围神经髓鞘形成。实验表明SBF2缺失导致施万细胞分化异常，与腓骨肌萎缩症(CMT4B2型)相关。
2. **文献名称**：*Structural Insights into SBF2 Protein: Role in Membrane Trafficking*
**作者**：J. Park et al.
**摘要**：通过X射线晶体学解析重组SBF2蛋白的PH结构域，揭示其特异性结合磷脂酰肌醇的分子机制，提出SBF2通过调控内体运输参与神经元膜修复过程。
3. **文献名称**：*Development of an SBF2 Knockout Mouse Model Using CRISPR/Cas9*
**作者**：L. Chen et al.
**摘要**：构建SBF2基因敲除小鼠模型，发现重组SBF2蛋白外源补充可部分恢复周围神经传导功能，证明其在维持轴突-施万细胞相互作用中的必要性。
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**注**：SBF2(SET Binding Factor 2)在真实文献中通常与遗传性周围神经病变相关，实际研究中可能涉及基因突变、蛋白互作或疾病机制。建议通过PubMed或Google Scholar以“SBF2 protein”、“MTMR13/SBF2 complex”等关键词检索最新文献。
**Background of Recombinant Human SBF2 Protein**
Recombinant human SBF2 (SET Binding Factor 2) protein is a genetically engineered version of the native SBF2 protein, which plays a critical role in cellular processes, particularly in myelination and peripheral nerve function. The SBF2 gene, located on chromosome 9. encodes a member of the pseudophosphatase family. Unlike typical phosphatases, SBF2 lacks enzymatic activity due to mutations in its catalytic domain, suggesting a regulatory or scaffolding role in signaling pathways.
SBF2 is primarily associated with the formation and maintenance of myelin sheaths in Schwann cells. Mutations in the SBF2 gene are linked to Charcot-Marie-Tooth disease type 4B2 (CMT4B2), a severe autosomal recessive neuropathy characterized by abnormal myelin folding and progressive muscle weakness. Recombinant SBF2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality. It serves as a vital tool for studying molecular mechanisms underlying myelination, nerve repair, and disease pathology. Researchers utilize this protein to investigate protein-protein interactions, screen therapeutic agents, and explore gene therapy strategies targeting inherited neuropathies. Its applications extend to drug development and biomarker studies for neurodegenerative disorders.
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