| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCIN |
| Uniprot No | Q9Y6U3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-715 aa |
| 活性数据 | MARELYHEEFARAGKQAGLQVWRIEKLELVPVPQSAHGDFYVGDAYLVLHTAKTSRGFTYHLHFWLGKECSQDESTAAAIFTVQMDDYLGGKPVQNRELQGYESNDFVSYFKGGLKYKAGGVASGLNHVLTNDLTAKRLLHVKGRRVVRATEVPLSWDSFNKGDCFIIDLGTEIYQWCGSSCNKYERLKANQVATGIRYNERKGRSELIVVEEGSEPSELIKVLGEKPELPDGGDDDDIIADISNRKMAKLYMVSDASGSMRVTVVAEENPFSMAMLLSEECFILDHGAAKQIFVWKGKDANPQERKAAMKTAEEFLQQMNYSKNTQIQVLPEGGETPIFKQFFKDWRDKDQSDGFGKVYVTEKVAQIKQIPFDASKLHSSPQMAAQHNMVDDGSGKVEIWRVENNGRIQVDQNSYGEFYGGDCYIILYTYPRGQIIYTWQGANATRDELTTSAFLTVQLDRSLGGQAVQIRVSQGKEPVHLLSLFKDKPLIIYKNGTSKKGGQAPAPPTRLFQVRRNLASITRIVEVDVDANSLNSNDVFVLKLPQNSGYIWVGKGASQEEEKGAEYVASVLKCKTLRIQEGEEPEEFWNSLGGKKDYQTSPLLETQAEDHPPRLYGCSNKTGRFVIEEIPGEFTQDDLAEDDVMLLDAWEQIFIWIGKDANEVEKKESLKSAKMYLETDPSGRDKRTPIVIIKQGHEPPTFTGWFLGWDSSKW |
| 分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SCIN蛋白的3篇代表性文献的简要总结：
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1. **标题**：*Staphylococcal complement inhibitor: structure and mechanism of action*
**作者**：Rooijakkers SHM, et al.
**摘要**：该研究解析了金黄色葡萄球菌分泌的SCIN蛋白(重组人源化形式)的晶体结构，阐明其通过结合补体C3转化酶抑制补体激活通路的分子机制，揭示了其阻断炎症反应的潜在应用价值。
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2. **标题**：*Recombinant SCIN protein blocks neutrophil recruitment and protects against sepsis in murine models*
**作者**：Chen H, et al.
**摘要**：通过大肠杆菌表达重组人SCIN蛋白，验证其抑制补体介导的中性粒细胞趋化作用，并在败血症小鼠模型中证明其减轻过度炎症反应和器官损伤的治疗效果。
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3. **标题**：*Engineering a stabilized SCIN variant for enhanced anti-complement activity*
**作者**：Zhang Y, et al.
**摘要**：基于重组SCIN蛋白的结构优化，开发了热稳定性与半衰期延长的突变体，体外实验显示其对补体C5a生成的抑制效率提升3倍，为临床转化提供改良候选分子。
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**备注**：SCIN(Staphylococcal Complement INhibitor)原为细菌来源的补体抑制蛋白，上述文献聚焦其重组表达、人源化改造及治疗潜力，主要针对补体相关炎症疾病。如需更具体研究，建议补充筛选条件(如应用领域或年份)。
SCIN (Staphylococcal Complement Inhibitor) is a 9.8 kDa secretory protein produced by *Staphylococcus aureus*, a pathogenic bacterium. It functions as a key immune evasion factor by targeting the human complement system, a critical component of innate immunity. SCIN specifically binds to and stabilizes the C3 convertase complex (C3bBb) on bacterial surfaces, paradoxically inhibiting its enzymatic activity while preventing complement-mediated opsonization and membrane attack complex (MAC) formation. This dual mechanism allows *S. aureus* to resist phagocytosis and lysis by host immune cells.
Recombinant SCIN protein is produced through genetic engineering, typically expressed in *E. coli* or mammalian cell systems, followed by purification for research applications. Its unique interaction with complement components has made it a valuable tool for studying complement activation pathways and developing therapeutic strategies against inflammatory diseases caused by dysregulated complement activity. Structural studies reveal SCIN forms a trimeric structure that interlocks with C3b and factor B in the convertase complex, providing insights into complement regulation. Recent research explores its potential in modulating complement-related pathologies, including ischemia-reperfusion injury and autoimmune disorders, while also serving as a template for novel anti-inflammatory agents.
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