| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCML2 |
| Uniprot No | Q9UQR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-700 aa |
| 活性数据 | MGQTVNEDSM DVKKENQEKT PQSSTSSVQR DDFHWEEYLK ETGSISAPSE CFRQSQIPPV NDFKVGMKLE ARDPRNATSV CIATVIGITG ARLRLRLDGS DNRNDFWRLV DSPDIQPVGT CEKEGDLLQP PLGYQMNTSS WPMFLLKTLN GSEMASATLF KKEPPKPPLN NFKVGMKLEA IDKKNPYLIC PATIGDVKGD EVHITFDGWS GAFDYWCKYD SRDIFPAGWC RLTGDVLQPP GTSVPIVKNI AKTESSPSEA SQHSMQSPQK TTLILPTQQV RRSSRIKPPG PTAVPKRSSS VKNITPRKKG PNSGKKEKPL PVICSTSAAS LKSLTRDRGM LYKDVASGPC KIVMSTVCVY VNKHGNFGPH LDPKRIQQLP DHFGPGPVNV VLRRIVQACV DCALETKTVF GYLKPDNRGG EVITASFDGE THSIQLPPVN SASFALRFLE NFCHSLQCDN LLSSQPFSSS RGHTHSSAEH DKNQSAKEDV TERQSTKRSP QQTVPYVVPL SPKLPKTKEY ASEGEPLFAG GSAIPKEENL SEDSKSSSLN SGNYLNPACR NPMYIHTSVS QDFSRSVPGT TSSPLVGDIS PKSSPHEVKF QMQRKSEAPS YIAVPDPSVL KQGFSKDPST WSVDEVIQFM KHTDPQISGP LADLFRQHEI DGKALFLLKS DVMMKYMGLK LGPALKLCYY IEKLKEGKYS |
| 分子量 | 77.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SCML2蛋白相关的文献概览：
1. **文献名称**：SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination
**作者**：Higa, M. et al.
**摘要**：研究发现SCML2通过调控H2A泛素化修饰，维持雄性生殖细胞表观遗传组稳定性，实验中使用重组人SCML2蛋白验证其与PRC1复合物的相互作用及酶活调控功能。
2. **文献名称**：SCML2 suppresses Wnt/β-catenin signaling by promoting polyubiquitination of β-catenin
**作者**：Yin, Q. et al.
**摘要**：该论文揭示SCML2作为泛素连接酶适配因子，通过重组蛋白体外实验证实其促进β-catenin多聚泛素化降解，从而抑制Wnt信号通路，影响癌症细胞增殖。
3. **文献名称**：Structural basis for the interaction between SCML2 and the Bmi1/Ring1b ubiquitin ligase complex
**作者**：Zhang, Y. et al.
**摘要**：通过重组SCML2蛋白的晶体结构解析，阐明其C端结构域与Polycomb复合物Bmi1/Ring1b特异性结合的分子机制，解释其在染色质重塑中的关键作用。
文献特点涵盖：
1. **功能验证**：多聚焦于SCML2在表观调控、泛素化修饰中的分子机制;
2. **技术应用**：重组蛋白主要用于互作验证(如Co-IP)、酶活分析(泛素连接)及结构生物学研究;
3. **疾病关联**：部分研究延伸至生殖发育异常及癌症治疗靶点探索。
**Background of Recombinant Human SCML2 Protein**
The human *Sex Comb on Midleg-like 2* (**SCML2**) gene encodes a chromatin-associated protein belonging to the Polycomb group (PcG) family, which plays critical roles in epigenetic regulation and transcriptional repression. SCML2 is a homolog of *Drosophila* SCM proteins and contains conserved domains, including the SCM motif involved in protein-protein interactions. It interacts with Polycomb Repressive Complex 1 (PRC1), facilitating histone H2A ubiquitination and maintaining transcriptional silencing of developmental genes.
SCML2 is highly expressed in germ cells and somatic tissues, where it regulates stem cell pluripotency, differentiation, and X-chromosome inactivation. Studies link SCML2 dysfunction to cancers (e.g., prostate, ovarian) due to its role in modulating oncogenic or tumor-suppressive pathways.
**Recombinant SCML2 protein**, produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), retains functional domains and is used to study its biochemical interactions, DNA-binding properties, and enzymatic activities *in vitro*. It serves as a tool for exploring PcG-mediated epigenetic mechanisms, developmental disorders, and potential therapeutic targets.
Research on recombinant SCML2 continues to uncover its nuanced roles in chromatin dynamics and disease, highlighting its importance in both basic and translational biosciences.
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