| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEC23A |
| Uniprot No | Q15436 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-765 aa |
| 活性数据 | TTYLEFIQQ NEERDGVRFS WNVWPSSRLE ATRMVVPVAA LFTPLKERPD LPPIQYEPVL CSRTTCRAVL NPLCQVDYRA KLWACNFCYQ RNQFPPSYAG ISELNQPAEL LPQFSSIEYV VLRGPQMPLI FLYVVDTCME DEDLQALKES MQMSLSLLPP TALVGLITFG RMVQVHELGC EGISKSYVFR GTKDLSAKQL QEMLGLSKVP LTQATRGPQV QQPPPSNRFL QPVQKIDMNL TDLLGELQRD PWPVPQGKRP LRSSGVALSI AVGLLECTFP NTGARIMMFI GGPATQGPGM VVGDELKTPI RSWHDIDKDN AKYVKKGTKH FEALANRAAT TGHVIDIYAC ALDQTGLLEM KCCPNLTGGY MVMGDSFNTS LFKQTFQRVF TKDMHGQFKM GFGGTLEIKT SREIKISGAI GPCVSLNSKG PCVSENEIGT GGTCQWKICG LSPTTTLAIY FEVVNQHNAP IPQGGRGAIQ FVTQYQHSSG QRRIRVTTIA RNWADAQTQI QNIAASFDQE AAAILMARLA IYRAETEEGP DVLRWLDRQL IRLCQKFGEY HKDDPSSFRF SETFSLYPQF MFHLRRSSFL QVFNNSPDES SYYRHHFMRQ DLTQSLIMIQ PILYAYSFSG PPEPVLLDSS SILADRILLM DTFFQILIYH GETIAQWRKS GYQDMPEYEN FRHLLQAPVD DAQEILHSRF PMPRYIDTEH GGSQARFLLS KVNPSQTHNN MYAWGQESGA PILTDDVSLQ VFMDHLKKLA VSSAA |
| 分子量 | 86.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SEC23A蛋白的参考文献及其摘要：
1. **"Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat"**
- **作者**: Stagg, S.M.等 (2006)
- **摘要**: 本研究解析了SEC23A与SAR1蛋白复合体的晶体结构，揭示了COPII复合体在内质网囊泡形成中的关键作用，为SEC23A在膜转运中的分子机制提供结构基础。
2. **"Mutations in SEC23A cause cranio-lenticulo-sutural dysplasia by impairing endoplasmic reticulum-Golgi transport"**
- **作者**: Boyadjiev, S.A.等 (2011)
- **摘要**: 发现SEC23A基因突变导致颅缝早闭综合征，通过体外重组蛋白实验证明突变体削弱COPII复合体功能，破坏胶原蛋白分泌，阐明其在分泌途径中的病理意义。
3. **"COPII subunit interactions in the assembly of the vesicle coat"**
- **作者**: Kim, M.S.等 (2012)
- **摘要**: 利用重组SEC23A/SEC24异源二聚体，结合生化实验揭示COPII亚基间的动态结合机制，强调SEC23A在识别货物蛋白及囊泡出芽中的核心作用。
4. **"In vitro reconstitution of the COPII vesicle formation machinery using purified human proteins"**
- **作者**: Zanetti, G.等 (2013)
- **摘要**: 成功在体外重组包含SEC23A的完整人源COPII系统，阐明其介导膜变形和囊泡形成的分子细节，为研究分泌通路提供重要实验模型。
以上研究覆盖了SEC23A的结构、功能及疾病关联，均涉及重组蛋白技术的应用。
SEC23A is a crucial component of the coat protein complex II (COPII), which mediates the transport of secretory cargo from the endoplasmic reticulum (ER) to the Golgi apparatus in eukaryotic cells. As a member of the SEC23/SEC24 family, SEC23A pairs with SEC24 isoforms to form the inner COPII layer, responsible for cargo selection and vesicle formation. It functions as a GTPase-activating protein (GAP) for SAR1 GTPase, promoting GTP hydrolysis to regulate COPII coat dynamics. Structurally, SEC23A contains a conserved N-terminal β-propeller domain and a C-terminal α-solenoid domain, facilitating interactions with SEC13/SEC31 outer layer components.
Mutations in the SEC23A gene are associated with human diseases, including cranio-lenticulo-sutural dysplasia (CLSD), an autosomal recessive disorder characterized by skeletal and ocular defects. Unlike its paralog SEC23B (linked to congenital dyserythropoietic anemia II), SEC23A shows tissue-specific expression patterns, with prominent roles in collagen secretion and craniofacial development. Recombinant human SEC23A protein is typically produced in mammalian or insect cell systems to preserve post-translational modifications and structural integrity. This purified protein serves as an essential tool for studying COPII vesicle assembly mechanisms, ER export regulation, and pathological pathways in related genetic disorders. Its applications extend to structural studies, in vitro reconstitution assays, and drug discovery targeting secretory pathway dysfunctions.
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