| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFRS9 |
| Uniprot No | Q13242 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-221 aa |
| 活性数据 | MSGWADERGGEGDGRIYVGNLPTDVREKDLEDLFYKYGRIREIELKNRHGLVPFAFVRFEDPRDAEDAIYGRNGYDYGQCRLRVEFPRTYGGRGGWPRGGRNGPPTRRSDFRVLVSGLPPSGSWQDLKDHMREAGDVCYADVQKDGVGMVEYLRKEDMEYALRKLDDTKFRSHEGETSYIRVYPERSTSYGYSRSRSGSRGRDSPYQSRGSPHYFSPFRPY |
| 分子量 | 51.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SFRS9蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**: **"Purification and characterization of human SFRS9 (SRp30c), a splicing factor involved in alternative splicing regulation"**
**作者**: Huang Y et al.
**摘要**: 该研究纯化并鉴定了重组表达的SFRS9蛋白，发现其通过特异性识别pre-mRNA中的富含丝氨酸/精氨酸结构域(RS domain)参与剪接调控，揭示了其在剪接体组装中的关键作用。
2. **文献名称**: **"Functional analysis of the SR protein SFRS9 in development and disease"**
**作者**: Manley JL, Tacke R.
**摘要**: 研究利用重组SFRS9蛋白进行功能实验，证明其在小鼠胚胎发育中调节神经相关基因的剪接，并与自闭症谱系障碍相关的剪接异常有潜在关联。
3. **文献名称**: **"SFRS9 interacts with DAZAP1 to modulate HIV-1 Rev-dependent viral RNA export"**
**作者**: Li H, et al.
**摘要**: 通过重组SFRS9蛋白与DAZAP1的互作研究发现，SFRS9可能通过结合病毒Rev蛋白调控HIV-1 RNA的核质转运，揭示了其在病毒感染中的新功能。
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(注：以上文献标题和作者为示例性虚构，实际需根据具体研究补充准确信息。建议通过PubMed或Google Scholar以“SFRS9”、“SRp30c”、“recombinant splicing factor”等关键词检索近期论文。)
**Background of Recombinant Human SFRS9 (SRSF9) Protein**
Serine/arginine-rich splicing factor 9 (SFRS9 or SRSF9) is a member of the serine/arginine (SR)-rich protein family, which plays critical roles in RNA splicing, a process essential for generating mature mRNA from precursor RNA. These proteins contain characteristic RNA recognition motifs (RRMs) and serine/arginine-rich domains, enabling them to interact with spliceosome components and regulate splice site selection. SRSF9 is ubiquitously expressed and localizes to nuclear speckles, dynamic subcellular structures involved in RNA processing.
Functionally, SRSF9 promotes constitutive splicing and modulates alternative splicing events, impacting gene expression diversity. It also participates in other RNA-related processes, including nuclear export, stability, and translation. Dysregulation of SRSF9 has been linked to diseases such as cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
Recombinant human SRSF9 protein is produced using engineered expression systems (e.g., *E. coli* or mammalian cells*)*, ensuring high purity and activity for experimental applications. It serves as a vital tool for studying splicing mechanisms, protein-RNA interactions, and disease mechanisms. Its availability accelerates research into therapeutic strategies targeting splicing dysregulation, emphasizing its relevance in molecular biology and biomedicine.
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