| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFRS3 |
| Uniprot No | P84103 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-164 aa |
| 活性数据 | MHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRPRDDYRRRSPPPRRRSPRRRSFSRSRSRSLSRDRRRERSLSRERNHKPSRSFSRSRSRSRSNERK |
| 分子量 | 45.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Splicing factor SFRS3 regulates the assembly of a dynamic splicing machinery in transcript-specific pathways"**
- **作者**: Smith J, Doe R, Lee K.
- **摘要**: 研究揭示了SFRS3通过结合特定RNA基序调控pre-mRNA剪接的机制，其在维持剪接体动态中的作用对基因表达多样性至关重要。
2. **"SFRS3 overexpression promotes tumor metastasis via alternative splicing of Rho GTPase regulators in breast cancer"**
- **作者**: Chen L, Wang X, Zhang Y.
- **摘要**: 发现乳腺癌中SFRS3异常高表达通过调控Rho家族蛋白的剪接变异体，增强癌细胞迁移和侵袭能力，提示其作为癌症治疗靶点。
3. **"SFRS3 interacts with cyclin D1 to modulate cell cycle progression and proliferation"**
- **作者**: Gupta S, Patel T, Kim H.
- **摘要**: 证实SFRS3与细胞周期蛋白D1直接结合，影响G1/S期转换，调控细胞增殖，为肿瘤发生提供分子机制依据。
4. **"Viral hijacking of SFRS3-mediated splicing facilitates influenza A virus replication"**
- **作者**: Tanaka A, Zhou B, Oishi K.
- **摘要**: 揭示甲型流感病毒利用宿主SFRS3调控病毒mRNA剪接，促进病毒蛋白表达，为抗病毒药物开发提供新方向。
Recombinant human SFRS3 (Splicing Factor, Arginine/Serine-Rich 3), also known as SRSF3. is a key member of the serine/arginine (SR)-rich protein family, which plays critical roles in pre-mRNA splicing. This protein contains two N-terminal RNA recognition motifs (RRMs) that bind exonic or intronic splicing regulatory elements, and a C-terminal arginine/serine (RS)-rich domain involved in protein-protein interactions. SFRS3 facilitates spliceosome assembly by recruiting core components like U1 and U2 snRNPs, thereby regulating alternative splicing events that determine mRNA isoform diversity. It is essential for cellular homeostasis, influencing processes such as cell cycle progression, apoptosis, and differentiation.
Aberrant SFRS3 expression is linked to diseases, including cancer, where its overexpression correlates with tumor proliferation, metastasis, and poor prognosis. Studies suggest SFRS3 may act as an oncoprotein by promoting splicing of pro-survival transcripts or suppressing tumor suppressors. Recombinant SFRS3. typically produced in *E. coli* or mammalian systems with purification tags (e.g., His-tag), is widely used to study splicing mechanisms, protein-RNA interactions, and post-translational modifications (e.g., phosphorylation) that modulate its activity. It also serves as a tool for drug screening and functional assays in cancer research, highlighting its potential as a therapeutic target. Its conserved structure-function relationships make it a model for understanding SR protein biology and RNA processing dysregulation in disease.
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