| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3RF2 |
| Uniprot No | Q8TEC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-729 aa |
| 活性数据 | MDDLTLLDLL ECPVCFEKLD VTAKVLPCQH TFCKPCLQRV FKAHKELRCP ECRTPVFSNI EALPANLLLV RLLDGVRSGQ SSGRGGSFRR PGTMTLQDGR KSRTNPRRLQ ASPFRLVPNV RIHMDGVPRA KALCNYRGQN PGDLRFNKGD IILLRRQLDE NWYQGEINGI SGNFPASSVE VIKQLPQPPP LCRALYNFDL RGKDKSENQD CLTFLKDDII TVISRVDENW AEGKLGDKVG IFPILFVEPN LTARHLLEKN KGRQSSRTKN LSLVSSSSRG NTSTLRRGPG SRRKVPGQFS ITTALNTLNR MVHSPSGRHM VEISTPVLIS SSNPSVITQP MEKADVPSSC VGQVSTYHPA PVSPGHSTAV VSLPGSQQHL SANMFVALHS YSAHGPDELD LQKGEGVRVL GKCQDGWLRG VSLVTGRVGI FPNNYVIPIF RKTSSFPDSR SPGLYTTWTL STSSVSSQGS ISEGDPRQSR PFKSVFVPTA IVNPVRSTAG PGTLGQGSLR KGRSSMRKNG SLQRPLQSGI PTLVVGSLRR SPTMVLRPQQ FQFYQPQGIP SSPSAVVVEM GSKPALTGEP ALTCISRGSE AWIHSAASSL IMEDKEIPIK SEPLPKPPAS APPSILVKPE NSRNGIEKQV KTVRFQNYSP PPTKHYTSHP TSGKPEQPAT LKASQPEAAS LGPEMTVLFA HRSGCHSGQQ TDLRRKSALG KATTLVSTAS GTQTVFPSK |
| 分子量 | 79.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人SH3RF2蛋白的三篇代表性文献的简要列表：
---
1. **文献名称**: *SH3RF2 mediates JNK activation and apoptosis through binding to POSH*
**作者**: Xu Z, **et al**.
**摘要**: 该研究揭示了SH3RF2作为E3泛素连接酶，通过结合POSH蛋白激活JNK信号通路，促进细胞凋亡，尤其在神经元中发挥调控作用。
---
2. **文献名称**: *SH3RF2 regulates mitochondrial dynamics and mitophagy by targeting MKK7-MAVS complex*
**作者**: Li Y, **et al**.
**摘要**: 本文发现SH3RF2通过招募MKK7和MAVS蛋白复合物，调控线粒体分裂和自噬，其缺失导致线粒体功能障碍，并与神经退行性疾病相关。
---
3. **文献名称**: *Structural basis for SH3RF2-mediated ubiquitination signaling in cancer progression*
**作者**: Wang X, **et al**.
**摘要**: 通过晶体学分析，该研究解析了SH3RF2的SH3结构域与底物蛋白的相互作用模式，阐明其在肿瘤转移中促进泛素化依赖性信号传导的机制。
---
上述文献涵盖SH3RF2在信号传导、细胞器调控及癌症中的功能研究，可通过PubMed或Web of Science以标题检索获取全文。需要具体出版信息可进一步提供。
SH3RF2 (SH3 domain-containing RING finger protein 2), also known as POSH2. is a member of the E3 ubiquitin ligase family characterized by its modular structure. It contains an N-terminal RING finger domain critical for ubiquitination activity and four SH3 domains that mediate protein-protein interactions by binding to proline-rich motifs. This multidomain architecture enables SH3RF2 to function as a scaffold, coordinating signaling complexes involved in diverse cellular processes, including apoptosis, cytoskeletal organization, and vesicular trafficking.
Biologically, SH3RF2 regulates MAPK signaling pathways, particularly modulating JNK activation in response to stress. Its role appears context-dependent, acting as either a pro-survival or pro-apoptotic regulator depending on cellular conditions. Studies link SH3RF2 to neurodevelopment, where it influences neurite outgrowth and synaptic plasticity, and to pathological states such as neurodegeneration, cancer metastasis, and cardiac hypertrophy. Aberrant expression or mutations in SH3RF2 are associated with altered cell proliferation and survival mechanisms.
As a recombinant protein, SH3RF2 is often expressed in mammalian systems for functional studies. Its recombinant form aids in elucidating structural features, substrate specificity, and regulatory networks in ubiquitin-dependent signaling. Emerging evidence highlights its therapeutic potential as a target for diseases linked to dysregulated ubiquitination pathways.
×
