| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC11A2 |
| Uniprot No | P49281 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCFSFRKLWAFTGPGFLMSIAYLDPGNIESDLQSGAVAGFKLLWILLLATLVGLLLKRLAARLGVVTGLHLAEVCHRQYPKVPRVILWLMVELAIIGSDMQEVIGSAIAINLLSVGRIPLWGGVLITIADTFVFLFLDKYGLRKLEAFFGFLITIMALTFGYEYVTVKPSQSQVLKGMFVPSCSGCRTPQIEQAVGIVGAVIMPHNMYLHSALVKSRQVNRNNKQEVREANKYFFIESCIALFVSFIINVFVVSVFAEAFFGKTNEQVVEVCTNTSSPHAGLFPKDNSTLAVDIYKGGVVLGCYFGPAALYIWAVGILAAGQSSTMTGTYSGQFVMEGFLNLKWSRFARVVLTRSIAIIPTLLVAVFQDVEHLTGMNDFLNVLQSLQLPFALIPILTFTSLRPVMSDFANGLGWRIAGGILVLIICSINMYFVVVYVRDLGHVALYVVAAVVSVAYLGFVFYLGWQCLIALGMSFLDCGHTVSISKGLLTEEATRGYVK |
| 分子量 | 61.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC11A2(DMT1)蛋白的3篇关键文献摘要：
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1. **标题**：**"Cloning and characterization of a mammalian proton-coupled metal-ion transporter"**
**作者**：Gunshin, H., et al. (1997)
**摘要**：该研究首次克隆并鉴定了SLC11A2(DMT1)，证明其作为质子偶联的跨膜转运蛋白，介导铁(Fe²⁺)和其他二价金属离子的摄入，对肠道铁吸收及细胞内铁代谢至关重要。
2. **标题**：**"Pathogenic mutations in the iron transporter DMT1 (SLC11A2) cause hypochromic microcytic anemia"**
**作者**：Fleming, M.D., et al. (2005)
**摘要**：研究发现SLC11A2基因突变会导致人类和小鼠的缺铁性贫血，揭示了DMT1在红系细胞铁摄取中的关键作用，并阐明其缺陷引发铁代谢紊乱的分子机制。
3. **标题**：**"Mechanisms of DMT1 function in iron transport and its role in neurological disorders"**
**作者**：Shawki, A., et al. (2015)
**摘要**：文章系统总结了SLC11A2的结构与功能，重点讨论其在中枢神经系统铁稳态中的调控作用，以及突变或表达异常与神经退行性疾病(如帕金森病)的潜在关联。
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**补充说明**：SLC11A2/DMT1的研究多聚焦于其转运铁、锰等金属的机制及其在贫血、神经病变中的病理意义。如需更多文献或特定研究方向，可进一步提供关键词。
SLC11A2 (solute carrier family 11 member 2), also known as DMT1 (divalent metal transporter 1), is a transmembrane protein crucial for cellular iron homeostasis. Encoded by the SLC11A2 gene in humans, it belongs to the SLC11/NRAMP family of proton-coupled divalent metal ion transporters. The protein primarily facilitates the uptake of ferrous iron (Fe²⁺) across cell membranes, functioning in duodenal iron absorption, endosomal iron export during transferrin-mediated uptake, and iron recycling in macrophages. Structurally, it contains 12 predicted transmembrane domains with N- and C-termini located intracellularly.
Recombinant human SLC11A2 protein is engineered using heterologous expression systems (e.g., HEK293 or insect cells) to study its transport mechanisms, structure-function relationships, and interactions with other proteins. Research highlights its pH-dependent activity, requiring a proton gradient to drive metal ion translocation. Mutations in SLC11A2 are linked to hypochromic microcytic anemia (Type IV) and neurodegenerative disorders due to disrupted iron metabolism. The recombinant form enables biochemical characterization, drug screening for iron-related disorders, and development of therapeutic strategies targeting iron metabolism pathways. Studies using purified SLC11A2 have clarified its substrate selectivity (Fe²⁺, Zn²⁺, Mn²⁺, Cd²⁺) and regulation by hypoxia, inflammatory signals, and post-translational modifications.
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