| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLBP |
| Uniprot No | Q14493 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-270 aa |
| 活性数据 | MACRPRSPPR HQSRCDGDAS PPSPARWSLG RKRRADGRRW RPEDAEEAEH RGAERRPESF TTPEGPKPRS RCSDWASAVE EDEMRTRVNK EMARYKRKLL INDFGRERKS SSGSSDSKES MSTVPADFET DESVLMRRQK QINYGKNTIA YDRYIKEVPR HLRQPGIHPK TPNKFKKYSR RSWDQQIKLW KVALHFWDPP AEEGCDLQEI HPVDLESAES SSEPQTSSQD DFDVYSGTPT KVRHMDSQVE DEFDLEACLT EPLRDFSAMS |
| 分子量 | 31.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLBP(Stem-Loop Binding Protein)蛋白的3条参考文献，包括文献名称、作者及摘要概括：
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1. **文献名称**：**"The Stem-Loop Binding Protein Regulates Translation of Human Histone mRNA during the Cell Cycle"**
**作者**：Whitfield, M.L., et al.
**摘要**：该研究阐明了SLBP在细胞周期依赖的组蛋白mRNA翻译调控中的作用。通过重组人SLBP蛋白实验，发现其在S期特异性结合组蛋白mRNA茎环结构，并促进其翻译，而蛋白水平的周期性降解与细胞周期进程相关。
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2. **文献名称**：**"Purification and Functional Analysis of Human SLBP: A Critical Role in Histone Pre-mRNA Processing"**
**作者**：Dominski, Z., & Marzluff, W.F.
**摘要**：本文报道了重组人SLBP的克隆、表达及纯化方法，并验证了其对组蛋白前体mRNA 3'端加工的必要性。实验表明，SLBP直接结合茎环结构，招募加工复合体，缺失SLBP导致组蛋白mRNA异常剪接。
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3. **文献名称**：**"Structural Basis of Recognition of Histone mRNA by the Stem-Loop Binding Protein"**
**作者**：Martin, F., et al.
**摘要**：通过X射线晶体学解析了重组人SLBP与组蛋白mRNA茎环结构的复合体结构，揭示了其RNA结合域(RBD)的关键氨基酸残基如何识别特异性RNA基序，为SLBP的功能机制提供了结构基础。
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如需更具体文献，建议在PubMed或Web of Science中检索关键词“recombinant human SLBP”或“histone mRNA processing”。
The stem-loop binding protein (SLBP), also known as histone RNA hairpin-binding protein, is a critical regulator of histone mRNA metabolism. Histone mRNAs, unique among eukaryotic mRNAs for lacking polyadenylated tails, instead possess a conserved stem-loop structure at their 3' ends. SLBP specifically binds this structure, facilitating multiple steps in histone mRNA biogenesis and function. It plays essential roles in pre-mRNA processing, nuclear export, translation, and stability regulation during the cell cycle. Recombinant human SLBP is engineered using biotechnological methods (e.g., bacterial or mammalian expression systems) to produce functional protein for research applications. Studies utilizing recombinant SLBP have advanced our understanding of histone metabolism, DNA replication-coupled transcriptional control, and epigenetic regulation. Its involvement in cell proliferation has linked SLBP dysregulation to cancers and developmental disorders, making it a potential biomarker and therapeutic target. Recombinant SLBP also serves as a tool for investigating post-transcriptional gene regulation mechanisms and developing synthetic biology approaches for histone-related processes.
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