| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC12A1 |
| Uniprot No | Q13621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430 aa |
| 活性数据 | MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEALRKQGASPLPQGAQSRRRRQTSLI |
| 分子量 | 72.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SLC12A1蛋白的参考文献示例(注：部分为模拟文献，实际引用时请核实数据库)：
1. **"Functional analysis of recombinant human SLC12A1 (NKCC2) in a heterologous expression system"**
*Authors: Gimenez, I., et al.*
**摘要**：本研究在HEK293细胞中成功表达重组人SLC12A1蛋白，通过放射性同位素标记和电生理学实验，证实其具有钠-钾-氯共转运活性，并发现特定磷酸化位点对其功能调节的关键作用。
2. **"Structural insights into the human NKCC2 transporter by cryo-EM"**
*Authors: Liu, Y., & Zhou, Q.*
**摘要**：利用冷冻电镜解析重组人SLC12A1蛋白的高分辨率结构，揭示其跨膜结构域中离子结合位点和Bartter综合征相关突变位点的空间分布，为疾病机制提供分子基础。
3. **"A novel SLC12A1 mutation impairs the trafficking of recombinant NKCC2 to the plasma membrane"**
*Authors: Watanabe, S., et al.*
**摘要**：构建携带Bartter综合征突变的重组人SLC12A1蛋白，发现突变导致蛋白在内质网滞留无法转运至细胞膜，功能实验表明突变体丧失离子转运能力。
4. **"High-throughput screening of SLC12A1 modulators using a fluorescent membrane potential assay"**
*Authors: Chen, L., et al.*
**摘要**：开发基于重组人SLC12A1蛋白的荧光筛选平台，鉴定了多种小分子化合物对转运体活性的抑制或增强作用，为利尿药物研发提供新靶点。
**提示**：实际文献需通过PubMed/Google Scholar检索关键词“recombinant human SLC12A1”“NKCC2 expression”或结合疾病关键词(如Bartter syndrome)获取。
**Background of Recombinant Human SLC12A1 Protein**
The **solute carrier family 12 member 1 (SLC12A1)**, also known as the **sodium-potassium-chloride cotransporter 2 (NKCC2)**, is a key membrane protein involved in ion transport across cell membranes. It belongs to the SLC12 family of electroneutral cation-coupled chloride cotransporters and plays a critical role in renal electrolyte homeostasis. NKCC2 is predominantly expressed in the thick ascending limb of the loop of Henle in the kidney, where it facilitates the reabsorption of sodium (Na⁺), potassium (K⁺), and chloride (Cl⁻) ions from the filtrate into the bloodstream. This process is essential for maintaining body fluid balance, blood pressure regulation, and urine concentration.
Genetic mutations in *SLC12A1* are associated with **Bartter syndrome type 1**, a rare autosomal recessive disorder characterized by salt wasting, hypokalemia, and metabolic alkalosis. The recombinant human SLC12A1 protein is produced via heterologous expression systems (e.g., mammalian cells or *E. coli*) for functional studies, drug screening, and therapeutic research. Its structural and functional characterization aids in understanding transport mechanisms, pharmacodynamics of loop diuretics (e.g., furosemide), and potential therapies for renal or hypertension-related diseases. Recombinant variants also serve as tools to investigate SLC12A1’s regulatory interactions with kinases, such as WNKs (With-No-Lysine kinases), critical for ion transport modulation.
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