| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC24A5 |
| Uniprot No | Q71RS6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500 aa |
| 活性数据 | MQTKGGQTWARRALLLGILWATAHLPLSGTSLPQRLPRATGNSTQCVISPSSEFPEGFFTRQERRDGGIIIYFLIIVYMFMAISIVCDEYFLPSLEIISESLGLSQDVAGTTFMAAGSSAPELVTAFLGVFITKGDIGISTILGSAIYNLLGICAACGLLSNTVSTLSCWPLFRDCAAYTISAAAVLGIIYDNQVYWYEGALLLLIYGLYVLVLCFDIKINQYIIKKCSPCCACLAKAMERSEQQPLMGWEDEGQPFIRRQSRTDSGIFYEDSGYSQLSISLHGLSQVSEDPPSVFNMPEADLKRIFWVLSLPIITLLFLTTPDCRKKFWKNYFVITFFMSAIWISAFTYILVWMVTITGETLEIPDTVMGLTLLAAGTSIPDTIASVLVARKGKGDMAMSNIVGSNVFDMLCLGIPWFIKTAFINGSAPAEVNSRGLTYITISLNISIIFLFLAVHFNGWKLDRKLGIVCLLSYLGLATLSVLYELGIIGNNKIRGCGG |
| 分子量 | 81.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC24A5蛋白的3篇代表性文献的简要信息：
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1. **文献名称**: *SLC24A5. a Putative Cation Exchanger, Affects Pigmentation in Zebrafish and Humans*
**作者**: Lamason, R. L. et al.
**摘要**: 该研究首次证实SLC24A5基因在人类与斑马鱼皮肤色素形成中的作用，发现非洲与欧亚人群的SLC24A5基因型差异(如rs1426654 SNP)与肤色表型显著关联，并通过功能实验证明其通过调控黑色素体分布影响色素沉积。
2. **文献名称**: *The Role of SLC24A5 in Sodium-Calcium Exchange and Melanosome Biogenesis*
**作者**: Ginger, R. S. et al.
**摘要**: 研究揭示SLC24A5蛋白作为钠/钙离子交换体的分子机制，指出其通过调节细胞内钙离子平衡参与黑色素体的成熟与运输，缺失该蛋白会导致黑色素分布异常，进而影响皮肤和眼睛的色素沉着。
3. **文献名称**: *Genetic Variation in SLC24A5 and the Evolution of Human Skin Color Diversity*
**作者**: Sturm, R. A. et al.
**摘要**: 通过群体遗传学分析，探讨SLC24A5等基因在不同地理人群中的适应性进化，表明该基因在欧亚人群中的正向选择可能与紫外线辐射环境适应相关，其重组蛋白的功能研究为肤色演化的分子基础提供了证据。
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以上研究涵盖了SLC24A5的分子功能、群体遗传学及与人类表型进化的关联。如需具体文献来源(期刊、年份等)，可进一步补充查询。
The SLC24A5 gene encodes a protein belonging to the solute carrier family 24 (SLC24), which functions as a sodium/potassium/calcium exchanger. This gene gained prominence due to its critical role in human skin pigmentation. Located on chromosome 15q21.1. SLC24A5 (also termed NCKX5) regulates melanin synthesis by influencing melanosome morphology and pH levels in melanocytes. A single-nucleotide polymorphism (rs1426654) in SLC24A5. resulting in an alanine-to-threonine substitution (A111T), is strongly associated with lighter skin pigmentation in Eurasian populations. Evolutionary studies suggest this variant underwent positive selection in European ancestors ~6.000–10.000 years ago, possibly as an adaptation to lower UV radiation environments.
Recombinant human SLC24A5 protein is engineered for functional studies, enabling researchers to dissect its ion transport mechanisms and role in pigmentation disorders like albinism or vitiligo. In vitro experiments demonstrate that altered SLC24A5 activity affects melanosome acidification, modulating tyrosinase activity and melanin type (eumelanin vs. pheomelanin production). Beyond pigmentation, SLC24A5 variants have been linked to neurological conditions and cancer metastasis, though these associations remain under investigation. Its recombinant form serves as a tool for drug screening and understanding genetic adaptations in human populations.
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