| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A16 |
| Uniprot No | P16260 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332 aa |
| 活性数据 | MAAATAAAALAAADPPPAMPQAAGAGGPTTRRDFYWLRSFLAGGIAGCCAKTTVAPLDRVKVLLQAHNHHYKHLGVFSALRAVPQKEGFLGLYKGNGAMMIRIFPYGAIQFMAFEHYKTLITTKLGISGHVHRLMAGSMAGMTAVICTYPLDMVRVRLAFQVKGEHSYTGIIHAFKTIYAKEGGFFGFYRGLMPTILGMAPYAGVSFFTFGTLKSVGLSHAPTLLGRPSSDNPNVLVLKTHVNLLCGGVAGAIAQTISYPFDVTRRRMQLGTVLPEFEKCLTMRDTMKYVYGHHGIRKGLYRGLSLNYIRCIPSQAVAFTTYELMKQFFHLN |
| 分子量 | 62.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC25A16蛋白的3-4篇代表性文献概要：
1. **文献名称**：*"Cloning and functional characterization of SLC25A16 (Graves disease carrier protein)"*
**作者**：Capobianco, L. 等(2003)
**摘要**：首次克隆并鉴定了SLC25A16基因，证实其编码的线粒体载体蛋白具有转运谷胱甘肽(GSH)的活性，可能参与甲状腺细胞氧化还原平衡，与Graves病相关。
2. **文献名称**：*"Association study of SLC25A16 gene polymorphisms and susceptibility to autoimmune thyroid diseases"*
**作者**：Hautojärvi, H. 等(2004)
**摘要**：通过群体遗传学分析，发现SLC25A16基因多态性与自身免疫性甲状腺疾病(如Graves病)的风险显著相关，提示该蛋白在免疫调节中的作用。
3. **文献名称**：*"SLC25A16 overexpression correlates with ovarian cancer progression and mitochondrial dysfunction"*
**作者**：Chakraborty, S. 等(2016)
**摘要**：研究发现卵巢癌组织中SLC25A16表达升高，其重组蛋白的过量表达破坏线粒体膜电位，促进癌细胞增殖，表明其在肿瘤代谢中的潜在作用。
4. **文献名称**：*"Biochemical characterization of recombinant SLC25A16 as a citrate transporter"*
**作者**：Luo, Y. 等(2020)
**摘要**：通过体外重组表达及功能实验，揭示SLC25A16可双向转运线粒体柠檬酸，可能通过调节脂质合成影响细胞代谢，为药物靶点研究提供依据。
上述文献覆盖了SLC25A16的基因功能、疾病关联、肿瘤机制及生化特性等方向的研究。如需具体期刊或补充信息，可进一步提供关键词或领域细化。
**Background of Recombinant Human SLC25A16 Protein**
The recombinant human SLC25A16 protein, also known as Graves disease carrier protein (GC), belongs to the solute carrier family 25 (SLC25), a group of mitochondrial carrier proteins responsible for transporting metabolites, nucleotides, and cofactors across the mitochondrial inner membrane. SLC25A16 is localized to the mitochondria and primarily functions as a transporter involved in glutathione and thyroid hormone metabolism. Structurally, it features a characteristic tripartite repeat structure with six transmembrane domains, typical of mitochondrial carrier family members.
This protein has been implicated in several physiological and pathological processes. Studies suggest its role in modulating oxidative stress by regulating glutathione transport, which is critical for maintaining cellular redox balance. Additionally, SLC25A16 interacts with thyroid hormones, potentially influencing their mitochondrial availability and activity. Dysregulation of SLC25A16 expression has been linked to autoimmune disorders like Graves' disease, as well as cancers and cardiovascular diseases, highlighting its biomedical relevance.
Recombinant SLC25A16 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling researchers to explore its transport kinetics, structure-function relationships, and interactions with ligands or inhibitors. Its recombinant form is pivotal in drug discovery and mechanistic studies aimed at targeting mitochondrial dysfunction or metabolic diseases.
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