| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC44A2 |
| Uniprot No | Q8IWA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706 aa |
| 活性数据 | MGDERPHYYGKHGTPQKYDPTFKGPIYNRGCTDIICCVFLLLAIVGYVAVGIIAWTHGDPRKVIYPTDSRGEFCGQKGTKNENKPYLFYFNIVKCASPLVLLEFQCPTPQICVEKCPDRYLTYLNARSSRDFEYYKQFCVPGFKNNKGVAEVLRDGDCPAVLIPSKPLARRCFPAIHAYKGVLMVGNETTYEDGHGSRKNITDLVEGAKKANGVLEARQLAMRIFEDYTVSWYWIIIGLVIAMAMSLLFIILLRFLAGIMVWVMIIMVILVLGYGIFHCYMEYSRLRGEAGSDVSLVDLGFQTDFRVYLHLRQTWLAFMIILSILEVIIILLLIFLRKRILIAIALIKEASRAVGYVMCSLLYPLVTFFLLCLCIAYWASTAVFLSTSNEAVYKIFDDSPCPFTAKTCNPETFPSSNESRQCPNARCQFAFYGGESGYHRALLGLQIFNAFMFFWLANFVLALGQVTLAGAFASYYWALRKPDDLPAFPLFSAFGRALRYHTGSLAFGALILAIVQIIRVILEYLDQRLKAAENKFAKCLMTCLKCCFWCLEKFIKFLNRNAYIMIAIYGTNFCTSARNAFFLLMRNIIRVAVLDKVTDFLFLLGKLLIVGSVGILAFFFFTHRIRIVQDTAPPLNYYWVPILTVIVGSYLIAHGFFSVYGMCVDTLFLCFLEDLERNDGSAERPYFMSSTLKKPLNKTNKKAAES |
| 分子量 | 106.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人SLC44A2蛋白的参考文献(内容为模拟概括)：
1. **"Structural insights into human SLC44A2 choline transporter-like protein"**
- Bickmeyer et al., 2018
- 通过X射线晶体学解析了重组人SLC44A2的跨膜结构，揭示其胆碱转运的潜在分子机制，提出其与神经系统疾病的关联性。
2. **"SLC44A2 regulates platelet activation and thrombosis risk via choline uptake"**
- Renaud et al., 2015
- 研究重组SLC44A2蛋白在血小板中的表达，发现其通过调控胆碱内流影响血小板活化，并验证了其基因突变与静脉血栓风险的关联。
3. **"Role of SLC44A2 in inflammatory pathways and hearing loss"**
- Schlossbauer et al., 2017
- 利用重组蛋白模型证实SLC44A2在内耳毛细胞中的功能，发现其缺失导致胆碱代谢异常，可能与遗传性耳聋有关。
4. **"SLC44A2 as a therapeutic target in neutrophil-driven thrombosis"**
- Giovannone et al., 2020
- 通过重组SLC44A2蛋白功能阻断实验，证明其介导中性粒细胞胞外陷阱(NETs)释放，提出抑制该蛋白可减轻病理性血栓形成。
注：上述文献为示例性概括，具体内容需以实际发表论文为准。建议通过PubMed或Google Scholar以关键词"SLC44A2"+"recombinant"+"function"检索最新研究。
Here’s a concise overview of recombinant human SLC44A2 protein in natural English:
SLC44A2 (Solute Carrier Family 44 Member 2), also known as CTL2 (choline transporter-like protein 2), is a transmembrane protein encoded by the *SLC44A2* gene. It belongs to the SLC44 family of solute carriers, which facilitate the transport of choline and other organic cations across cellular membranes. SLC44A2 is notably expressed in mitochondria, plasma membranes, and secretory vesicles, playing roles in choline metabolism, lipid membrane synthesis, and cellular signaling.
Research highlights its involvement in pathophysiological processes, including platelet activation, inflammatory responses, and autoimmune conditions. SLC44A2 has been linked to diseases like Alzheimer’s due to its choline transport function in neurons and to cancer progression in certain malignancies (e.g., prostate, colorectal) where its overexpression is observed. The protein’s extracellular domain also carries the Jr(a)/HNA-3b blood group antigen, making it clinically relevant in transfusion-related acute lung injury (TRALI).
Recombinant human SLC44A2 is produced via heterologous expression systems (e.g., HEK293 cells) for functional studies, antibody development, and drug discovery. Its purified form enables research into its structure, transport mechanisms, and therapeutic targeting in choline-related disorders or cancer.
(Word count: ~230)
×
