| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC40A1 |
| Uniprot No | Q9NP59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571 aa |
| 活性数据 | MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSANIVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFRFAQNTLGNKLFACGPDAKEVRKENQANTSVV |
| 分子量 | 88.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC40A1(Ferroportin)蛋白的3篇代表性参考文献的示例(基于研究领域典型内容，部分为假设性概括)：
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1. **文献名称**: *Structure of the human ferroportin bound to hepcidin reveals mechanisms of iron transport regulation*
**作者**: Zhang, X., et al.
**摘要**:
本研究通过冷冻电镜解析了人源重组SLC40A1蛋白与铁调节激素hepcidin的复合物结构，揭示了hepcidin通过结合Ferroportin引发其内吞降解的分子机制，为遗传性血色素沉着症的治疗提供结构基础。
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2. **文献名称**: *Functional characterization of ferroportin mutations linked to iron overload disorders*
**作者**: Nemeth, E., et al.
**摘要**:
作者利用重组表达的SLC40A1突变体，在细胞模型中分析其铁转运功能及对hepcidin的敏感性，发现特定突变(如A77D)导致Ferroportin蛋白铁外排能力缺陷，并解析了突变引发铁代谢异常的病理机制。
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3. **文献名称**: *Optimized expression and purification of recombinant human ferroportin in HEK293 cells*
**作者**: Tang, Y., et al.
**摘要**:
文章报道了人源SLC40A1蛋白在哺乳动物HEK293细胞中的高效重组表达与纯化方法，并通过表面等离子体共振(SPR)验证其与hepcidin的结合活性，为后续功能研究提供可靠蛋白样品。
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**注**：以上文献为示例性质，实际引用请通过PubMed/Google Scholar检索最新论文。如需具体文章，可提供关键词进一步筛选。
Ferroportin (SLC40A1), encoded by the *SLC40A1* gene, is the sole known iron exporter in humans, playing a critical role in systemic iron homeostasis. Expressed primarily in cell types responsible for iron release—such as enterocytes (absorbing dietary iron), macrophages (recycling iron from senescent red blood cells), and hepatocytes (storing iron)—ferroportin facilitates the transport of intracellular iron into plasma, where it binds to transferrin for distribution to tissues. Its activity is tightly regulated by the hormone hepcidin, which binds to ferroportin, triggering its internalization and degradation, thereby reducing iron efflux. This interaction represents a key regulatory mechanism for maintaining iron balance.
Mutations in *SLC40A1* cause ferroportin disease, a type of hereditary hemochromatosis. Pathogenic variants are classified into two subtypes: loss-of-function mutations (type A) impair iron export, leading to iron overload in macrophages, while gain-of-function mutations (type B) confer resistance to hepcidin, causing excessive iron absorption and parenchymal iron deposition. Recombinant human ferroportin proteins are widely studied to dissect these mechanisms, resolve structural features (e.g., 12 transmembrane domains, iron-binding sites), and screen therapeutic compounds. Targeting ferroportin-hepcidin signaling holds promise for treating iron-related disorders, such as anemia of chronic disease (via hepcidin inhibitors) or iron overload syndromes (via hepcidin agonists). Additionally, recombinant ferroportin aids in drug development and understanding iron dysregulation in cancer and neurodegeneration.
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