| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPB |
| Uniprot No | P14678 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-80 aa |
| 活性数据 | MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSM |
| 分子量 | 34.54 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant human SNRPB expression and its role in spliceosome assembly"**
- **作者**: Smith J. et al.
- **摘要**: 研究通过大肠杆菌系统成功表达重组人SNRPB蛋白，并验证其参与pre-mRNA剪接体组装的分子机制，揭示了SNRPB与其他剪接因子的互作网络。
2. **"Structural insights into SNRPB protein-RNA interactions by cryo-EM"**
- **作者**: Li X. et al.
- **摘要**: 利用冷冻电镜解析了重组SNRPB与U1 snRNA复合体的三维结构，阐明了其RNA结合域的关键氨基酸位点及功能重要性。
3. **"SNRPB overexpression correlates with cancer progression and alternative splicing dysregulation"**
- **作者**: Garcia R. et al.
- **摘要**: 通过重组SNRPB蛋白的体外实验，发现其在多种癌细胞中异常高表达，导致特定肿瘤相关基因的异常剪接，促进肿瘤侵袭性。
4. **"Functional characterization of SNRPB mutations in congenital disorders"**
- **作者**: Wang Y. et al.
- **摘要**: 构建了SNRPB突变体重组蛋白并验证其功能缺失，证明其与先天性颅面发育异常(如CCFD综合征)的致病性关联。
(注：以上为模拟参考文献，实际文献需通过PubMed等数据库检索。)
SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B') is a core component of the Sm protein family, essential for pre-mRNA splicing. It plays a critical role in assembling small nuclear ribonucleoproteins (snRNPs), which are key subunits of the spliceosome. SNRPB binds to uridine-rich small nuclear RNAs (snRNAs) like U1. U2. U4. and U5. forming a stable ring-shaped structure with other Sm proteins (e.g., SNRPD1. SNRPE). This complex ensures proper snRNP biogenesis and spliceosome function, enabling precise recognition and excision of introns during mRNA maturation.
Mutations in the *SNRPB* gene are linked to developmental disorders, notably cerebrocostomandibular syndrome (CCMS) and some forms of autosomal dominant congenital cleft palate. These variants often disrupt protein structure, impairing snRNP assembly and splicing efficiency, which can lead to tissue-specific developmental defects. SNRPB is ubiquitously expressed but tightly regulated, with its dysregulation implicated in cancer progression and neurodegenerative diseases.
Recombinant SNRPB proteins are produced via bacterial (e.g., *E. coli*) or eukaryotic expression systems for functional studies. They serve as tools to investigate splicing mechanisms, model disease-associated mutations, and screen for therapeutic agents targeting spliceosomal activity. Its conserved structure and central role in RNA processing make SNRPB a focal point in understanding genetic regulation and disease pathology.
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