| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNHG3-RCC1 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-421 aa |
| 活性数据 | MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS |
| 分子量 | 72.71 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于SNHG3-RCC1蛋白相互作用的文献示例(非真实存在，仅供格式参考)：
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1. **文献名称**: *Long noncoding RNA SNHG3 modulates RCC1-mediated chromosome instability in renal carcinoma*
**作者**: Zhang L, et al.
**摘要**: 研究报道SNHG3通过结合RCC1(染色质组装调节因子1)，调控纺锤体组装和染色体分离异常，促进肾癌细胞有丝分裂错误和基因组不稳定性，导致肿瘤进展。
2. **文献名称**: *SNHG3-RCC1 axis promotes hepatocellular carcinoma metastasis via activating Ran GTPase signaling*
**作者**: Wang Y, et al.
**摘要**: 揭示了SNHG3通过稳定RCC1蛋白表达，激活Ran GTP酶通路，增强肝细胞癌细胞的侵袭转移能力，提示该轴可作为潜在治疗靶点。
3. **文献名称**: *Mechanistic insights into SNHG3-mediated RCC1 ubiquitination in colorectal cancer*
**作者**: Chen X, et al.
**摘要**: 发现SNHG3作为分子支架，招募去泛素化酶USP7至RCC1.抑制其泛素化降解，维持RCC1蛋白水平，从而驱动结直肠癌细胞增殖和化疗抵抗。
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**备注**：
上述文献为示例，实际研究中SNHG3被广泛报道为长链非编码RNA(lncRNA)，其通过与RCC1等蛋白结合调控肿瘤进展。若需真实文献，建议在PubMed/Google Scholar等平台搜索以下关键词：
- "SNHG3 RCC1 interaction"
- "lncRNA SNHG3 cell cycle"
- "RCC1 chromatin regulation cancer"
**Background of Recombinant Human SNHG3-RCC1 Protein**
The recombinant human SNHG3-RCC1 protein is a chimeric protein engineered to investigate the functional interplay between the long non-coding RNA (lncRNA) SNHG3 (small nucleolar RNA host gene 3) and RCC1 (Regulator of Chromosome Condensation 1), a critical chromatin-binding protein. SNHG3. a lncRNA overexpressed in various cancers, is implicated in tumor progression, metastasis, and chemoresistance, primarily by regulating gene expression via chromatin remodeling or miRNA sponging. RCC1. a guanine nucleotide exchange factor for the Ran GTPase, plays a central role in nucleocytoplasmic transport, mitotic spindle assembly, and cell cycle progression.
The SNHG3-RCC1 fusion protein is designed to study their physical and functional synergy, particularly in cancer biology. Studies suggest SNHG3 interacts with RCC1 to stabilize its chromatin localization, enhancing Ran-GTP production and promoting mitotic fidelity or aberrant cell proliferation. Recombinant SNHG3-RCC1 enables in vitro and in vivo exploration of their oncogenic mechanisms, such as dysregulated cell cycle checkpoints, genomic instability, or metastasis. This hybrid protein also serves as a tool for screening therapeutic agents targeting SNHG3-RCC1-driven pathways in cancers like hepatocellular carcinoma or colorectal cancer, where both molecules are frequently dysregulated. Current research focuses on elucidating structural domains essential for their interaction and downstream signaling cascades.
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