| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAPC5 |
| Uniprot No | O75971 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-98 aa |
| 活性数据 | MLSRLQELRK EEETLLRLKA ALHDQLNRLK VEELALQSMI SSRRGDEMLS SHTVPEQSHD MLVHVDNEAS INQTTLELST KSHVTEEEEE EEEEESDS |
| 分子量 | 11.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNAPC5蛋白的3篇代表性文献的示例(注：文献为示例性概括，具体细节需根据实际研究调整)：
1. **文献名称**：*Structural and functional analysis of the human SNAPC5 subunit in RNA Polymerase III transcription*
**作者**：Henry, T. et al.
**摘要**：通过重组表达和纯化SNAPC5蛋白，结合冷冻电镜技术解析其三维结构，揭示了SNAPC5在SNAPc复合体中与其他亚基的相互作用界面，并验证其招募RNA Pol III至启动子的关键功能。
2. **文献名称**：*Recombinant SNAPC5 enhances in vitro transcription of human U6 snRNA genes*
**作者**：Lee, J. & Hernandez, N.
**摘要**：构建并纯化重组SNAPC5蛋白，通过体外转录实验证实其与SNAPc其他亚基协同作用，显著提高RNA Pol III介导的U6 snRNA基因转录活性，为研究非编码RNA调控机制提供工具。
3. **文献名称**：*Dysregulation of SNAPC5 expression alters tRNA synthesis and promotes cancer cell proliferation*
**作者**：Smith, L. et al.
**摘要**：利用重组SNAPC5蛋白进行功能回补实验，发现其表达水平异常通过干扰tRNA合成影响肿瘤细胞翻译效率，进而调控细胞周期进程，提示SNAPC5在癌症中的潜在治疗价值。
如需具体文献，建议在PubMed或Google Scholar以关键词“SNAPC5 recombinant”“SNAPc complex RNA Pol III”检索最新研究。
**Background of Recombinant Human SNAPC5 Protein**
SNAPC5 (Small Nuclear RNA Activating Complex Polypeptide 5) is a critical subunit of the SNAPc (SNRNA Activating Protein Complex), a multi-subunit transcription factor essential for the expression of small nuclear RNA (snRNA) genes transcribed by RNA polymerase III (Pol III). This complex binds to the proximal sequence element (PSE) in snRNA gene promoters, facilitating the assembly of Pol III and coactivators to initiate transcription. SNAPC5 contributes to the stability and promoter recognition specificity of SNAPc, interacting with other subunits (e.g., SNAPC1. SNAPC3. SNAPC4) to form a functional DNA-binding domain.
Recombinant human SNAPC5 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for *in vitro* studies to dissect its role in snRNA biogenesis, transcriptional regulation, and interactions with other factors. Its recombinant form, often tagged with purification labels (e.g., His-tag), enables detailed biochemical and structural analyses, including investigations into mutations linked to developmental disorders or altered snRNA expression in diseases like cancer. Studies leveraging recombinant SNAPC5 enhance understanding of Pol III-driven transcription mechanisms and potential therapeutic targets.
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