| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMU1 |
| Uniprot No | Q2TAY7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-513 aa |
| 活性数据 | MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL QAIQSLKLPD KTLIDLYEQV VLELIELREL GRSLLRQT DPMIMLKQTQ PERYIHLENL LARSYFDPRE AYPDGSSKEK RRIAQALA GEVSVVPPSR LMALLGQALK WQQHQGLLPP GMTIDLFRGK VKDVEEEK FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS FVNEATFTQD GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV NSVILLPKNP EHFVVCNRSN TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL SPRGEWIYCV GEDFVLYCFS TVTGKLERTL TVHEKDVIGI AHHPHQNLIA TYSEDGLLKL WKP |
| 分子量 | 57.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMU1蛋白的3篇文献的简要摘要信息：
1. **文献名称**：*SMU1 interacts with the EXOSC10-RRP6-PDCD7 complex in pre-mRNA 3' end processing*
**作者**：Sun Y. et al. (2021)
**摘要**：研究利用重组人SMU1蛋白，揭示了其与EXOSC10-RRP6-PDCD7复合物的相互作用，证实SMU1通过调控mRNA 3'末端加工影响RNA代谢和细胞周期进程。
2. **文献名称**：*Smu1 facilitates the assembly of the spliceosome through regulating protein-RNA interactions*
**作者**：Liu Q. et al. (2019)
**摘要**：通过重组表达SMU1蛋白，发现其作为RNA结合蛋白参与剪接体早期组装，协调U1和U2 snRNP的结合，从而维持RNA剪接的精确性和效率。
3. **文献名称**：*SMU1 modulates apoptosis by regulating the stability of pro-apoptotic BCL2 family members*
**作者**：Kim T. et al. (2017)
**摘要**：该研究发现重组SMU1蛋白通过与BCL2家族凋亡蛋白(如BAX)相互作用，抑制其泛素化降解，增强细胞对凋亡信号的敏感性，提示SMU1在细胞存活中起关键作用。
*注：以上文献为示例性质，实际引用需核对真实出版物信息。*
Recombinant human SMU1 (Suppressor of Mec-8 and U-insertion 1) protein is a conserved RNA-binding protein implicated in multiple cellular processes, including mRNA splicing, cell cycle regulation, and stress response. SMU1 is a component of the spliceosome complex and interacts with the CDC5L complex, playing a role in pre-mRNA processing and alternative splicing. Studies suggest its involvement in maintaining genomic stability and modulating transcriptional fidelity. Structurally, SMU1 contains an N-terminal domain with unknown function and a C-terminal RNA recognition motif (RRM) essential for RNA interaction.
Dysregulation of SMU1 has been linked to diseases such as cancer and neurodegenerative disorders. For instance, altered SMU1 expression correlates with tumor progression and chemoresistance in certain cancers. Recombinant SMU1 protein, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., His-tag, FLAG-tag), is widely used to study its molecular mechanisms. It facilitates *in vitro* assays like RNA-protein interaction analysis, spliceosome assembly studies, and structural characterization. Ongoing research focuses on elucidating its role in splicing fidelity, cellular stress pathways, and potential therapeutic targeting. SMU1’s integration into diverse regulatory networks highlights its importance in both basic biology and disease contexts.
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