| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMEK1 |
| Uniprot No | Q6IN85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-833 aa |
| 活性数据 | MTDTRRRVKVYTLNEDRQWDDRGTGHVSSGYVERLKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVASSLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGIFLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEVIPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREFVMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLMGLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPSKDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHAFLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNSAIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS |
| 分子量 | 121.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMEK1蛋白的3篇代表性文献及摘要概括：
1. **文献名称**: *"SMEK1 is a regulator of PP4 phosphatase in mitotic progression"*
**作者**: Li et al.
**摘要**: 研究发现SMEK1通过与蛋白磷酸酶PP4复合体结合调控细胞周期，重组人SMEK1蛋白的过表达导致有丝分裂延迟，表明其在染色体排列和纺锤体检查点中的关键作用。
2. **文献名称**: *"Structural basis for PP4 interaction with SMEK1 in dephosphorylation signaling"*
**作者**: Wang et al.
**摘要**: 通过重组表达人SMEK1蛋白并结合X射线晶体学，揭示了SMEK1与PP4复合体的结合位点，阐明其对底物去磷酸化过程的分子机制，为癌症治疗提供潜在靶点。
3. **文献名称**: *"SMEK1 promotes oxidative stress resistance via PP4-mediated ATF2 activation"*
**作者**: Chen et al.
**摘要**: 研究利用重组SMEK1蛋白验证其通过激活PP4-ATF2信号通路增强细胞抗氧化应激能力，提示SMEK1在神经退行性疾病或衰老中的潜在调控功能。
如有其他需求可进一步补充!
Recombinant human SMEK1 (Suppressor of MEK1) protein, also known as PP4R3A or PPP4R3A, is a regulatory subunit of protein phosphatase 4 (PP4), a serine/threonine phosphatase involved in diverse cellular processes. SMEK1 forms a complex with the catalytic subunit PP4C, playing critical roles in regulating mitogen-activated protein kinase (MAPK) signaling pathways, DNA damage response, and cell cycle progression. It acts as a suppressor of MEK1-dependent MAPK activation by promoting PP4-mediated dephosphorylation of key signaling components, thereby modulating cell proliferation, differentiation, and apoptosis.
Structurally, SMEK1 contains conserved domains that facilitate PP4 assembly and substrate targeting. Recombinant versions are typically expressed in *E. coli* or mammalian systems, enabling biochemical studies to dissect its interaction networks and enzymatic regulation. Research highlights its involvement in maintaining genomic stability, oxidative stress responses, and neuronal development. Dysregulation of SMEK1 has been implicated in pathologies such as cancer, neurodegenerative disorders, and metabolic syndromes, making it a potential therapeutic target. Current studies focus on elucidating its structural-functional relationships and crosstalk with other phosphatases/kinases. The availability of recombinant SMEK1 protein accelerates mechanistic insights into PP4-mediated signaling and supports drug discovery efforts targeting phosphatase-associated diseases.
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