| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMARCE1 |
| Uniprot No | Q969G3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-411 aa |
| 活性数据 | MSKRPSYAPP PTPAPATQMP STPGFVGYNP YSHLAYNNYR LGGNPGTNSR VTASSGITIP KPPKPPDKPL MPYMRYSRKV WDQVKASNPD LKLWEIGKII GGMWRDLTDE EKQEYLNEYE AEKIEYNESM KAYHNSPAYL AYINAKSRAE LEEESRQR QSRMEKGEPY MSIQPAEDPD DYDDGFSMKH TATARFQRNH RLISEILSES VVPDVRSVVT TARMQVLKRQ VQSLMVHQRK LEAELLQIEE RHQEKKRKFL ESTDSFNNEL KRLCGLKVEV DMEKIEIA QAEEQARKRQ EEREKEEQ AERSQSSIVP EEEQNKGE EKKDDENIPM ETEETHLEET TESQQNGEEG TSTPEDKESG QEGVDSMAEE GTSDSNTGSE SNSATVEEPP TDPIPEDEKK E |
| 分子量 | 46.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人SMARCE1蛋白的参考文献示例(注：内容为虚构示例，实际文献需根据数据库检索补充)：
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1. **文献名称**: "Structural basis of SMARCE1 in chromatin remodeling"
**作者**: Patel, D. et al.
**摘要**: 本研究解析了SMARCE1蛋白与SWI/SNF复合体核心亚基结合的高分辨率结构，揭示了其通过调节组蛋白H2A-H2B动态调控染色质重塑的分子机制。
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2. **文献名称**: "SMARCE1 mutations drive epigenetic dysregulation in meningioma"
**作者**: Smith, L. et al.
**摘要**: 发现SMARCE1基因突变导致染色质可及性异常，与脑膜瘤的发生相关，突变的SMARCE1蛋白丧失对癌基因抑制的调控功能。
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3. **文献名称**: "SMARCE1 regulates DNA repair via BRG1-dependent pathways"
**作者**: Lee, H. et al.
**摘要**: 证明SMARCE1通过招募BRG1至DNA损伤位点，促进同源重组修复，其缺失显著增加细胞对电离辐射的敏感性。
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4. **文献名称**: "Functional interplay between SMARCE1 and histone chaperones"
**作者**: Brown, R. et al.
**摘要**: 揭示了SMARCE1与HIRA组蛋白伴侣协作，通过维持核小体稳定性影响胚胎干细胞多能性，为发育生物学提供新机制。
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实际文献建议通过PubMed或Web of Science以关键词“SMARCE1”、“chromatin remodeling”、“SWI/SNF complex”检索。
SMARCE1. a critical subunit of the SWI/SNF chromatin remodeling complex, plays a pivotal role in regulating gene expression by modifying chromatin structure. Encoded by the *SMARCE1* gene, this protein contains a SANT domain and a C-terminal SNF2-related region, enabling its interaction with DNA and other subunits to facilitate ATP-dependent nucleosome repositioning. The SWI/SNF complex governs cellular processes such as transcription, DNA repair, and differentiation by disrupting histone-DNA contacts, thereby making genomic regions accessible for transcriptional machinery.
Mutations in *SMARCE1* are linked to genetic disorders, including familial clear cell carcinoma and certain subtypes of brain tumors (e.g., spinal clear cell meningiomas), highlighting its tumor-suppressive functions. SMARCE1 also exhibits tissue-specific roles, influencing embryonic development and neural crest cell migration. Dysregulation of SMARCE1 is associated with cancer progression, metastasis, and therapy resistance due to altered chromatin dynamics.
Recombinant human SMARCE1 protein is commonly generated using bacterial or mammalian expression systems for functional studies. It serves as a tool to investigate chromatin remodeling mechanisms, protein-DNA interactions, and SWI/SNF complex assembly. Recent research focuses on its interplay with histone modifications (e.g., acetylation) and its potential as a therapeutic target for cancers with SWI/SNF deficiencies. Studies also explore its role in epigenetic reprogramming and cellular plasticity, emphasizing its broad impact on genomic stability and disease pathogenesis.
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