| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMARCD2 |
| Uniprot No | Q92925 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MSGRGAGGFP LPPLSPGGGA VALGAPPP PAGPGMLPGP ALRGPGPAGG VGGPGAFR PMGPAGPQ YQRPGMSPGN RMPMAGLQVG PPAGSPFG APLRPGMPPT MMDPFRKRLL VPQAQPPMPA QRRGLKRRKM ADKVLPQRIR ELVPESQAYM DLLAFERKLD QTIARKRMEI QEAIKKPLTQ KRKLRIYISN TFSPSKAEGD SAGTAGTPGG TPAGDKVASW ELRVEGKLLD DPSKQKRKFS SFFKSLVIEL DKELYGPDNH LVEWHRMPTT QETDGFQVKR PGDLNVKCTL LLMLDHQPPQ YKLDPRLARL LGVHTQTR IMQALWLYIK HNQLQDGHER EYINCNRYFR QIFSCGRLRF SEIPMKLAGL LQHPDPIVIN HVISVDPNDQ KKTACYDIDV EVDDPLKAQM SNFLASTTNQ QEIASLDVKI HETIESINQL KTQRDFMLSF STDPQDFIQE WLRSQRRDLK IITDVIGNPE EERRFYHQ PWAQEAVGRH IFAKVQQRRQ ELEQVLGIRL T |
| 分子量 | 58.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMARCD2蛋白的参考文献示例，涵盖其功能、结构及疾病相关性研究。请注意，以下内容为模拟示例，实际文献需通过学术数据库进一步核实：
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1. **标题**: *SMARCD2 regulates hematopoietic stem cell self-renewal through chromatin remodeling*
**作者**: Krasteva, V., et al.
**摘要**: 研究揭示了SMARCD2通过调控染色质重塑复合体BAF的组装，维持造血干细胞的自我更新能力。实验利用重组人SMARCD2蛋白进行功能回补，证实其通过激活关键基因(如Runx1)调控分化过程。
2. **标题**: *Structural and functional analysis of SMARCD2 in SWI/SNF complex assembly*
**作者**: Alver, B.H., et al.
**摘要**: 通过重组SMARCD2蛋白的体外结合实验和冷冻电镜技术，解析了SMARCD2与SMARCE1的相互作用界面，阐明其在SWI/SNF复合体中的支架作用，为突变相关疾病的机制提供依据。
3. **标题**: *SMARCD2 deficiency drives leukemogenesis via disrupted enhancer-mediated transcription*
**作者**: Holmes, Z.R., et al.
**摘要**: 发现SMARCD2缺失通过破坏增强子-启动子相互作用，激活致癌通路(如Wnt/β-catenin)。研究中使用重组SMARCD2蛋白恢复染色质可及性，逆转白血病细胞增殖表型。
4. **标题**: *SMARCD2 as a coactivator of androgen receptor in prostate cancer progression*
**作者**: Wang, Q., et al.
**摘要**: 证实SMARCD2通过重组蛋白实验增强雄激素受体(AR)的转录活性，促进前列腺癌细胞生长，为靶向SMARCD2-AR轴的治疗策略提供理论基础。
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建议通过PubMed或Google Scholar以“SMARCD2 recombinant protein”、“BAF complex SMARCD2”等关键词检索最新文献，以获取准确信息。
SMARCD2 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2) is a component of the SWI/SNF chromatin-remodeling complex, which regulates gene expression by modulating chromatin structure through ATP-dependent nucleosome repositioning. As part of the SMARCD/BAF60 family, SMARCD2 contains conserved domains for binding nuclear receptors and transcription factors, facilitating their recruitment to target gene promoters. It plays critical roles in cellular differentiation, development, and homeostasis, particularly in hematopoiesis, immune cell function, and neural development. Recombinant human SMARCD2 protein is typically produced using expression systems like *E. coli* or mammalian cells, often with purification tags (e.g., His-tag) to enable functional studies. Research links SMARCD2 mutations to human diseases, including primary immunodeficiencies and developmental disorders, underscoring its importance in immune regulation and epigenetic mechanisms. Current studies focus on its interactions with signaling pathways (e.g., TGF-β, Notch) and roles in cancer progression, where SWI/SNF complexes often exhibit tumor-suppressive functions. Recombinant SMARCD2 aids in elucidating structure-function relationships, screening for inhibitors/activators, and developing gene therapies targeting chromatin remodeling defects.
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