| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMC1L1 |
| Uniprot No | Q14683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 366-465 aa |
| 活性数据 | TLEENQVKKYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAKIKQKLREIEENQKRIEKLEEYITTSKQSLEEQKKLEGELTEEVEM |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMC1蛋白(注：可能存在名称差异，如"SMC1L1"可能指"SMC1A"或"SMC1B"，此处按SMC1相关研究整理)的3篇关键文献：
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1. **文献名称**: Cohesin: Its Roles and Mechanisms
**作者**: Nasmyth K, Haering CH
**摘要**: 系统综述了cohesin复合体的结构与功能，重点解析SMC1蛋白与SMC3形成复合体的分子机制，及其在姐妹染色单体粘连、染色体分离和DNA修复中的作用(Science, 2002)。
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2. **文献名称**: The structure and function of SMC proteins
**作者**: Hirano T
**摘要**: 通过结构生物学研究揭示了SMC家族(包括SMC1)的ATP酶活性结构域功能，阐明SMC1在染色体动态组装中的关键调控，并探讨其在基因组稳定性和细胞周期调控中的作用(Genes & Development, 2006)。
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3. **文献名称**: Shugoshin collaborates with SMC1 protein in meiotic chromosome cohesion
**作者**: Kitajima TS, et al.
**摘要**: 研究证明SMC1在减数分裂中与Shugoshin蛋白协同作用，保护姐妹染色单体的粘连复合体不被过早解离，揭示了其在生殖细胞发育中的特异性调控机制(Cell, 2004)。
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**备注**：若您的研究方向具体涉及重组蛋白表达技术，可补充：
4. **文献名称**: Expression and purification of recombinant human SMC1 for structural studies
**作者**: Gligoris TG, et al.
**摘要**: 报道了人源SMC1蛋白在大肠杆菌中的重组表达、纯化及结晶方法，为后续功能研究提供技术支撑(J Struct Biol, 2014)。
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建议根据具体研究需求补充"SMC1A/SMC1B"或"Cohesin复合体"等关键词进一步检索。
SMC1L1 (Structural Maintenance of Chromosomes 1-like 1), also known as SMC1A, is a key component of the cohesin complex, a multi-subunit protein machinery essential for chromosome organization and genomic stability. Belonging to the SMC protein family, SMC1A forms a heterodimer with SMC3. creating a V-shaped structure that interacts with RAD21 and STAG1/STAG2 to form a ring-like complex. This structure mediates sister chromatid cohesion during mitosis and meiosis, ensuring accurate chromosome segregation. Beyond its role in cohesion, SMC1A participates in DNA repair, transcriptional regulation, and chromatin architecture maintenance. Mutations in SMC1A are linked to Cornelia de Lange syndrome, a developmental disorder characterized by growth delays and craniofacial abnormalities, highlighting its critical role in embryonic development. Dysregulation of SMC1A has also been associated with cancer progression, as aberrant cohesin function can drive genomic instability. Recombinant SMC1L1 protein is commonly produced in expression systems (e.g., E. coli or mammalian cells) for in vitro studies to investigate its biochemical interactions, structural dynamics, and functional roles in chromatin organization. Its study provides insights into mechanisms underlying chromosomal disorders, DNA repair pathways, and potential therapeutic targets for cohesinopathies and malignancies.
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