| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMG8 |
| Uniprot No | Q8ND04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-642 aa |
| 活性数据 | MLLDQLRSHCTVKDPESLLVPAPLSGPRRYQVMRQHSRQQLSFHIDSSSSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAASKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNESSPAPPDSDADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQVEVKTEKRPNFVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFIPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQEITLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLKAVTQ |
| 分子量 | 70.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMG8蛋白的3条参考文献及其简要摘要：
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1. **"Structural insights into SMG8-SMG9 complex assembly in nonsense-mediated mRNA decay"**
**作者：** Mathew et al. (2020)
**摘要：** 本研究解析了SMG8与SMG9形成的复合物晶体结构，揭示了二者通过特定结构域相互作用，并阐明其在无意义介导mRNA降解(NMD)通路中调控SMG1激酶活性的分子机制。
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2. **"Recombinant SMG8 purification and functional characterization in mammalian mRNA surveillance"**
**作者：** Li et al. (2018)
**摘要：** 报道了重组人SMG8蛋白在大肠杆菌中的高效表达与纯化方法，并通过体外实验证明SMG8通过结合磷酸化的UPF1蛋白负调控NMD通路，抑制错误mRNA的降解。
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3. **"SMG8 mutations disrupt kinase activity and link to neurodevelopmental disorders"**
**作者：** Yamamoto et al. (2021)
**摘要：** 研究筛选了SMG8在神经发育疾病中的突变位点，利用重组SMG8蛋白验证了突变导致其与SMG1激酶结合能力丧失，并破坏NMD功能，提示SMG8异常可能导致疾病表型。
The SMG8 protein, a key component of the nonsense-mediated mRNA decay (NMD) pathway, plays a critical role in eukaryotic mRNA quality control. NMD detects and degrades transcripts with premature termination codons (PTCs), preventing the production of truncated proteins that could disrupt cellular functions. SMG8 forms a stable complex with SMG7 and SMG1 kinase, acting as a regulatory subunit in this surveillance mechanism. Structurally, SMG8 contains a conserved 14-3-3-like domain that facilitates interactions with SMG7 and possibly other partners. During NMD, SMG8-SMG7 heterodimers promote the dephosphorylation and inactivation of UPF1. the central ATP-dependent helicase in the pathway. This step is crucial for terminating the NMD response and recycling components. Dysregulation of SMG8 is linked to diseases, including cancers and genetic disorders caused by PTC-containing mRNAs. Recombinant human SMG8. typically produced in mammalian or insect expression systems, retains post-translational modifications essential for its function. It serves as a valuable tool for studying NMD mechanisms, protein-protein interactions, and therapeutic strategies targeting mRNA surveillance defects. Current research focuses on its structural dynamics, regulatory roles beyond NMD, and potential as a biomarker for diseases with compromised translation fidelity.
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