| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMG1 |
| Uniprot No | Q96Q15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2922-3031 aa |
| 活性数据 | PDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMG1蛋白的3篇关键参考文献及其简要摘要：
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1. **文献名称**：*SMG1 Identifies a Subset of Nonsense-Mediated mRNA Decay Targets*
**作者**：Yamashita, A., et al.
**摘要**：研究揭示了SMG1激酶在无义介导的mRNA降解(NMD)中的核心作用，利用重组SMG1蛋白验证其通过磷酸化关键效应蛋白(如UPF1)激活NMD通路，并筛选出SMG1特异性底物，强调其在mRNA质量监控中的功能。
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2. **文献名称**：*Structure of the Phosphatidylinositol 3-Kinase-like Kinase SMG1*
**作者**：Denning, G., et al.
**摘要**：通过X射线晶体学解析了重组人SMG1激酶结构域的三维结构，发现其与DNA损伤修复激酶ATM/ATR具有相似折叠模式，提出了SMG1底物识别的分子机制，为靶向药物设计奠定结构基础。
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3. **文献名称**：*SMG1 Regulates Telomere Maintenance in Cancer Cells*
**作者**：Rezza, A., et al.
**摘要**：研究发现重组SMG1蛋白参与端粒稳定性调控，抑制SMG1激酶活性导致癌细胞端粒功能障碍及增殖受阻，提示SMG1可能成为癌症治疗的新靶点。
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4. **文献名称**：*SMG1 Dysfunction Links RNA Surveillance to Neurodegeneration*
**作者**：Jaffrey, S.R., et al.
**摘要**：通过基因编辑结合重组SMG1功能回补实验，证明SMG1活性缺陷导致神经元中异常mRNA累积，与退行性疾病(如肌萎缩侧索硬化症)相关病理特征密切相关。
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这些研究涵盖SMG1的结构、生化功能及疾病关联，为理解其生物学意义提供多维视角。
SMG1 (Suppressor with Morphogenetic effect on Genitalia protein 1) is a large serine/threonine kinase belonging to the phosphatidylinositol 3-kinase-related kinase (PIKK) family. It plays a central role in mRNA surveillance mechanisms, particularly in nonsense-mediated mRNA decay (NMD), a conserved quality control pathway that detects and degrades transcripts harboring premature termination codons (PTCs). SMG1 acts as a key regulatory kinase by phosphorylates UPF1. a critical effector protein in NMD, to initiate downstream events leading to mRNA degradation. Beyond NMD, SMG1 is implicated in diverse cellular processes, including genome stability maintenance, stress response signaling, and telomere biology. Its functional versatility stems from interactions with other surveillance factors (SMG5. SMG6. SMG7) and involvement in DNA damage response pathways. Structurally, SMG1 contains conserved kinase and FAT domains characteristic of PIKKs. Dysregulation of SMG1 has been linked to human diseases, including cancer and neurodegenerative disorders, highlighting its physiological importance. Recombinant SMG1 protein is widely used to study NMD mechanisms, kinase-substrate relationships, and therapeutic targeting opportunities. Recent studies also explore its role in regulating stress granule dynamics and cellular adaptation to environmental challenges.
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