| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMYD1 |
| Uniprot No | Q8NB12 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490 aa |
| 活性数据 | MTIGRMENVE VFTAEGKGRG LKATKEFW DIIFAERAYS AVVFDSLVNF VCHTCFKRQE KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC SAIKRYGKVP NENIRLRI MWRVEREGTG LTEGCLVSVD DLQNHVEHFG EEEQKDLRVD VDTFLQYWPP QSQQFSMQYI SHIFGVINCN GFTLSDQRGL QAVGVGIFPN LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG KISEGEELTV SYIDFLNVSE ERKRQLKKQY YFDCTCEHCQ KKLKDDLFLG VKDNPKPSQE VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNIYM LRMLSIVSEV LSYLQAFEEA SFYARRMVDG YMKLYHPNNA QLGMAVMRAG LTNWHAGNIE VGHGMICKAY AILLVTHGPS HPITKDLEAM RVQTEMELRM FRQNEFMYYK MRELNNQP MQVMAEPSNE PSPALFHKKQ |
| 分子量 | 56.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SMYD1蛋白的3篇虚构参考文献示例(仅作模板参考，非真实文献)：
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1. **文献名称**：*Structural and Functional Insights into SMYD1 Lysine Methyltransferase Activity*
**作者**：Chen L. et al.
**摘要**：本研究解析了重组人SMYD1蛋白的晶体结构，揭示了其SET结构域与S-腺苷甲硫氨酸(SAM)辅因子的结合模式，并证实SMYD1对组蛋白H3K4的特异性甲基化修饰功能，为其在表观遗传调控中的作用提供机制基础。
2. **文献名称**：*SMYD1 Modulates Cardiac Development via Interaction with Muscle-specific Transcription Factors*
**作者**：Johnson R.B. et al.
**摘要**：通过体外重组SMYD1蛋白实验，发现其与心肌细胞分化关键因子(如GATA4)相互作用，促进心脏特异性基因表达。小鼠模型中SMYD1缺失导致心室发育异常，表明其在心脏形成中的必要性。
3. **文献名称**：*SMYD1 Regulates Skeletal Muscle Metabolism through PPARγ Coactivation*
**作者**：Gupta S. et al.
**摘要**：研究表明，重组SMYD1蛋白通过甲基化修饰PPARγ共激活因子，增强线粒体氧化代谢相关基因的转录，揭示SMYD1在骨骼肌能量稳态中的调控作用，并提示其与代谢疾病的潜在关联。
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如需真实文献，建议在PubMed或Google Scholar中以“SMYD1 recombinant protein”“human SMYD1 function”等关键词检索。
SMYD1 (SET and MYND domain-containing protein 1) is a member of the SMYD family of lysine methyltransferases, characterized by a conserved catalytic SET domain and a MYND domain involved in protein-protein interactions. Primarily expressed in cardiac and skeletal muscle tissues, SMYD1 plays a critical role in regulating muscle development, differentiation, and function. It catalyzes the methylation of histones (e.g., H3K4) and non-histone targets (e.g., sarcomeric proteins), influencing chromatin remodeling and gene transcription. Studies highlight its involvement in cardiac morphogenesis, sarcomere organization, and calcium handling in cardiomyocytes, with deficiencies linked to congenital heart defects and skeletal myopathies. SMYD1 also interacts with transcriptional regulators like HDACs and SRF, modulating muscle-specific gene expression. Recombinant human SMYD1 protein, produced via bacterial or eukaryotic expression systems, is widely used to study its enzymatic activity, substrate specificity, and structural properties in vitro. Its applications extend to drug discovery, particularly in identifying modulators for treating cardiovascular or muscular disorders. Despite progress, the full scope of SMYD1's biological roles, tissue-specific regulatory networks, and potential therapeutic relevance remain active areas of investigation. Ongoing research aims to clarify its context-dependent functions and validate its utility as a biomarker or intervention target in muscle-related pathologies.
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