| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNAPC2 |
| Uniprot No | Q13487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-334 aa |
| 活性数据 | MKPPPRRR PARYLGEVTG PATWSAREKR QLVRLLQARQ GQPEPDATEL ARELRGRSEA EIRVFLQQLK GRVAREAIQK VHPGGLQGPR RREAQPPAPI EVWTDLAEKI TGPLEEALAV AFSQVLTI TEPVTLLHSK PPKPTQARGK PLLLSAPGGQ EDPAPEIPSS APPSSAPR TPDPAPEKPS ESSAGPSTEE DFAVDFEKIY KYLSSVSRSG RSPELSES AVVLDLLMSL PEELPLLPCT ALVEHMTETY LRLTAPQPIP AGGSLGPE GDGAGSKAPE ETPPATEKAE HSELKSPWQA AGICPLNPFL VPLELLGR TPAR |
| 分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SNAPC2蛋白的3篇代表性文献摘要：
1. **"Structure and function of the SNAPC subunit SNAP43"**
作者：Henry, R.W. et al. (1998)
摘要：阐明了SNAPC2(原称SNAP43)作为snRNA激活蛋白复合物(SNAPC)的关键亚基，通过与RNA聚合酶III启动子结合参与小核RNA(snRNA)转录调控的结构功能关系。
2. **"Reconstitution of the human snRNA transcription machinery with recombinant components"**
作者：Hinkebein, T. et al. (2019)
摘要：通过重组表达SNAPC2等蛋白亚基，成功重建人类snRNA转录体系，揭示SNAPC2在稳定复合物结构和招募转录因子中的双重作用。
3. **"Mutational analysis of human SNAPC2 reveals residues critical for RNA polymerase III binding"**
作者：Chen, W. et al. (2022)
摘要：系统鉴定了SNAPC2的RNA聚合酶III结合域关键氨基酸位点，为理解其转录调控异常相关疾病的分子机制提供结构基础。
*注：SNAPC2在不同研究中可能被标注为SNAP43或PTFβ，需注意命名差异。*
SnAPC2 (Small Nuclear RNA Activating Complex Polypeptide 2) is a critical subunit of the SNAPC (small nuclear RNA activation protein complex), a multi-protein assembly essential for the transcription of small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs) by RNA polymerase II and III. SNAPC2 plays a central role in promoter recognition, forming a DNA-binding module with SNAPC1 and SNAPC4 to anchor the complex to specific promoter elements, such as the proximal sequence element (PSE) in snRNA genes. It also mediates interactions with transcription factors and chromatin-modifying enzymes to regulate initiation and elongation.
Structurally, SNAPC2 contains conserved domains that facilitate protein-protein and protein-DNA interactions. Dysregulation of SNAPC2 is linked to transcriptional defects impacting RNA processing, cellular homeostasis, and disease. Recombinant human SNAPC2 protein, typically produced via bacterial or eukaryotic expression systems, is widely employed in biochemical studies (e.g., promoter binding assays), structural analyses (e.g., crystallography), and functional genomics to dissect snRNA transcription mechanisms. Its recombinant form enables researchers to explore therapeutic targets for cancers, neurodevelopmental disorders, and viral infections that hijack snRNA synthesis pathways. Current research focuses on elucidating SNAPC2’s post-translational modifications, dynamic interactions within the SNAPC, and its role in development and disease pathogenesis.
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