| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPAG8 |
| Uniprot No | Q99932 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426 aa |
| 活性数据 | METNESTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA AAAAASAAAA TAAFTTAKAA ALSTKTPAPC SEFMEPSSDP SLLGEPCAGP GFTHNIAHGS LGFEPVYVSC IAQDTCTTTD HSSNPGPVPG SSSGPVLGSS SGAGHGSGSG SGPGCGSVPG SGSGPGPGSG PGSGPGHGSG SHPGPASGPG PDTGPDSELS PCIPPGFRNL VADRVPNYTS WSQHCPWEPQ KQPPWEFLQV LEPGARGLWK PPDIKGKLMV CYETLPRGQC LLYNWEEERA TNHLDQVPSM QDGSESFFFR HGHRGLLTMQ LKSPMPSSTT QKDSYQPPGN VYWPLRGKRE AMLEMLLQHQ ICKEVQAEQE PTRKLFEVES VTHHDYRMEL AQAGTPAPTK PHDYRQEQPE TFWIQRAPQL PVCEGD |
| 分子量 | 51.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据SPAG8蛋白研究常见方向模拟的参考文献示例(实际文献请通过PubMed等学术平台查询)：
**1. 文献名称**: "Molecular characterization and expression analysis of human SPAG8 in testicular tissues"
**作者**: Zhang Y, et al.
**摘要**: 首次克隆并表达了重组人SPAG8蛋白，证实其在睾丸组织中的高表达，可能与精子发生过程中的微管组装调控相关。
**2. 文献名称**: "SPAG8 as a novel biomarker for ovarian cancer: insights from recombinant protein-based assays"
**作者**: Gupta R, et al.
**摘要**: 通过重组SPAG8蛋白开发ELISA检测，发现其在卵巢癌患者血清中显著上调，提示其作为癌症诊断标志物的潜力。
**3. 文献名称**: "Structural insights into SPAG8-CEP164 interaction and its role in ciliogenesis"
**作者**: Lee S, et al.
**摘要**: 利用重组SPAG8蛋白进行结构解析，揭示其与CEP164蛋白的互作界面，为纤毛形成异常的分子机制提供新依据。
**4. 文献名称**: "SPAG8 deficiency disrupts acrosome formation in spermatids: evidence from knockout mouse models"
**作者**: Tanaka H, et al.
**摘要**: 构建SPAG8基因敲除小鼠模型，发现其精子顶体发育缺陷，证明该蛋白在雄性生育中的关键作用。
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**提示**：建议通过以下方式获取真实文献：
1. 访问 **PubMed** (https://pubmed.ncbi.nlm.nih.gov/) 搜索 "SPAG8" 或 "sperm-associated antigen 8"。
2. 在 **Google Scholar** 使用关键词 "recombinant human SPAG8 protein"。
3. 查阅《Biology of Reproduction》《Human Reproduction》等生殖生物学领域期刊。
**Background of Recombinant Human SPAG8 Protein**
Sperm-associated antigen 8 (SPAG8), also known as TSA1. is a protein encoded by the *SPAG8* gene in humans. It is primarily expressed in the testis and plays a critical role in spermatogenesis, particularly in regulating sperm development, motility, and function. Structurally, SPAG8 contains conserved domains associated with RNA binding, suggesting potential roles in post-transcriptional regulation during germ cell maturation. Aberrant SPAG8 expression has been linked to male infertility, as mutations or dysregulation may disrupt sperm formation or fertilization capacity.
The recombinant human SPAG8 protein is engineered using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to produce purified, biologically active forms for research. This allows studies into its molecular interactions, such as binding partners in sperm-egg recognition pathways, or its involvement in cellular stress responses via redox regulation. Emerging evidence also highlights its potential association with cancers, where SPAG8 may influence tumor proliferation or metastasis. Current research focuses on elucidating its mechanistic pathways, diagnostic applications in reproductive health, and therapeutic targeting. Recombinant SPAG8 serves as a vital tool for dissecting its physiological and pathological roles, bridging gaps in understanding gametogenesis and disease.
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