| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPACA1 |
| Uniprot No | Q9HBV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-294 aa |
| 活性数据 | MSPRGTGCSAGLLMTVGWLLLAGLQSARGTNVTAAVQDAGLAHEGEGEEETENNDSETAENYAPPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSVRYKDSTSLDQLPTEMPGEDDALSEWNE |
| 分子量 | 58.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人SPACA1蛋白相关的代表性文献摘要(注：文献标题与作者为模拟示例，供参考)：
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1. **《SPACA1-deficient mice are sterile with impaired sperm acrosome formation》**
*作者：Fujihara Y, et al.*
**摘要**：通过构建SPACA1基因敲除小鼠模型，发现该蛋白缺失导致雄性不育，精子顶体结构异常。利用重组SPACA1蛋白进行体外补充实验，证实其对顶体囊泡融合的关键作用。
2. **《Crystal structure of human SPACA1 reveals a conserved protein fold involved in gamete interaction》**
*作者：Ikawa M, et al.*
**摘要**：首次解析了重组人SPACA1蛋白的晶体结构，揭示其具有跨膜结构域和与配子结合相关的功能表面，为研究其在受精中的分子机制提供结构基础。
3. **《Autoantibodies against SPACA1 as a potential biomarker for male infertility》**
*作者：Yamaguchi R, et al.*
**摘要**：利用重组SPACA1蛋白进行ELISA检测，发现部分不明原因不育男性患者血清中存在抗SPACA1自身抗体，提示其可能作为免疫性不育的诊断标志物。
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*注：以上文献信息为基于领域知识的模拟内容，实际引用需检索具体数据库(如PubMed)并核实原文。建议以“SPACA1 recombinant protein”为关键词查找最新研究。*
SPACA1 (Sperm Acrosome Associated 1) is a protein-coding gene located on human chromosome 9q31.1. It encodes a glycoprotein predominantly expressed in the male reproductive system, particularly in spermatids during late spermiogenesis. The protein localizes to the acrosome, a lysosome-related organelle critical for sperm-egg fusion. Structurally, SPACA1 contains conserved cysteine-rich domains and N-glycosylation sites, suggesting roles in protein stability and extracellular interactions. Functionally, it participates in acrosome formation, sperm maturation, and fertilization by mediating membrane adhesion events. Studies in mice models indicate that SPACA1 deficiency leads to male infertility due to acrosomal malformation and impaired sperm penetration ability. Its dysregulation has been associated with human teratozoospermia and failed assisted reproductive outcomes. Recombinant human SPACA1 protein is typically produced in mammalian expression systems to maintain post-translational modifications. This engineered protein serves as a key reagent for investigating molecular mechanisms of fertility, developing diagnostic markers for male infertility, and screening potential therapeutic agents. Recent research also explores its ectopic expression in cancers, though reproductive biology remains its primary study focus.
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