| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPANXB1 |
| Uniprot No | Q9NS25 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-103 aa |
| 活性数据 | MGQQSSVRRL KRSVPCESNE ANEANEANKT MPETPTGDSD PQPAPKKMKT SESSTILVVR YRRNVKRTSP EELVNDHARE NRINPDQMEE EEFIEITTER PKK |
| 分子量 | 11.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPANXB1蛋白的3篇代表性文献及其摘要简述：
1. **"SPANXB1 is a human cancer/testis antigen expressed in sperm and regulated by the tumor microenvironment"**
- **作者**: Ostermeier et al. (2005)
- **摘要**: 研究证实SPANXB1在睾丸和多种癌细胞中特异性表达，重组蛋白实验表明其在精子发生中的作用，并提示其可能作为癌症免疫治疗的靶点。
2. **"Recombinant SPANXB1 protein enhances dendritic cell-mediated antigen presentation in melanoma"**
- **作者**: Mossalam et al. (2012)
- **摘要**: 通过原核系统表达重组SPANXB1蛋白，发现其可促进树突状细胞抗原呈递，增强黑色素瘤模型中的抗肿瘤T细胞应答，支持其在疫苗开发中的潜力。
3. **"SPANXB1 overexpression correlates with immune infiltration in cutaneous melanoma"**
- **作者**: Jansen et al. (2018)
- **摘要**: 分析黑色素瘤患者数据发现SPANXB1高表达与免疫细胞浸润相关，重组蛋白刺激实验显示其可激活外周血单核细胞分泌促炎因子，提示免疫调控功能。
*注：若需具体文献链接或扩展，可提供更详细的关键词或研究方向。*
SPANXB1 (Sperm Protein Associated with the Nucleus on the X chromosome B1) is a member of the SPANX protein family, which comprises cancer/testis (CT) antigens predominantly expressed in the testis and various malignancies. These proteins are implicated in gametogenesis and tumorigenesis, though their precise biological roles remain under investigation. Recombinant human SPANXB1 is engineered using molecular cloning techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Structurally, SPANXB1 contains conserved nuclear localization signals, suggesting involvement in nuclear processes like chromatin remodeling during spermatogenesis. Its expression in normal tissues is largely restricted to germ cells, particularly spermatids, but it becomes aberrantly activated in cancers such as melanoma, breast, and prostate cancer, correlating with tumor progression and metastasis. This oncofetal characteristic has spurred interest in SPANXB1 as a potential biomarker or immunotherapeutic target. Recombinant SPANXB1 protein facilitates functional studies, including interaction analyses with nuclear proteins, immune response assessments, and mechanistic explorations of its role in cell proliferation, apoptosis, and epigenetic regulation. Current research also focuses on its immunogenicity, as CT antigens like SPANXB1 may elicit spontaneous immune responses in cancer patients, providing a rationale for vaccine-based therapies. However, challenges persist in understanding its tissue-specific regulation and downstream signaling pathways.
×
