| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SS18 |
| Uniprot No | Q15532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-418aa |
| 活性数据 | SVAFAAPRQ RGKGEITPAA IQKMLDDNNH LIQCIMDSQN KGKTSECSQY QQMLHTNLVY LATIADSNQN MQSLLPAPPT QNMPMGPGGM NQSGPPPPPR SHNMPSDGMV GGGPPAPHMQ NQMNGQMPGP NHMPMQGPGP NQLNMTNSSM NMPSSSHGSM GGYNHSVPSS QSMPVQNQMT MSQGQPMGNY GPRPNMSMQP NQGPMMHQQP PSQQYNMPQG GGQHYQGQQP PMGMMGQVNQ GNHMMGQRQI PPYRPPQQGP PQQYSGQEDY YGDQYSHGGQ GPPEGMNQQY YPDGHNDYGY QQPSYPEQGY DRPYEDSSQH YYEGGNSQYG QQQDAYQGPP PQQGYPPQQQ QYPGQQGYPG QQQGYGPSQG GPGPQYPNYP QGQGQQYGGY RPTQPGPPQP PQQRPYGYDQ GQYGNYQQ |
| 分子量 | 45.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SS18蛋白的3条参考文献示例，涵盖不同研究方向：
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1. **文献名称**: *Structural insights into the SS18-SSX fusion oncoprotein in synovial sarcoma*
**作者**: Crew AJ, et al.
**摘要**: 该研究解析了重组SS18-SSX融合蛋白的晶体结构，揭示其通过破坏BAF染色体重塑复合物的正常组装，促进致癌基因异常转录的分子机制。
2. **文献名称**: *SS18 regulates human transcription via interaction with the BAF complex*
**作者**: Su L, et al.
**摘要**: 利用重组人SS18蛋白进行体外结合实验，证明其作为BAF复合物的核心组分，通过招募染色质修饰酶调控胚胎发育和肿瘤发生中的关键基因表达。
3. **文献名称**: *Targeting SS18-SSX dependency in synovial sarcoma using recombinant protein-based drug screening*
**作者**: Bishop JA, et al.
**摘要**: 研究构建了重组SS18-SSX蛋白的高通量筛选平台，鉴定出可破坏融合蛋白与BAF复合物相互作用的小分子抑制剂，为滑膜肉瘤治疗提供潜在策略。
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*注：以上为模拟参考文献，实际文献需通过PubMed/Google Scholar等平台检索确认。建议结合关键词“SS18 recombinant protein”、“SS18-SSX fusion”以及“BAF complex”查找最新研究。*
**Background of SS18 Protein**
The SS18 protein, encoded by the *SS18* gene located on human chromosome 18. is a nuclear transcriptional regulator implicated in chromatin remodeling and transcriptional co-activation. Structurally, it contains a SYT N-terminal homology (SNH) domain and a C-terminal SSXT domain, facilitating interactions with transcriptional coactivators and chromatin-modifying complexes. SS18 is a critical component of the BAF (SWI/SNF) chromatin remodeling complex, which regulates gene expression by altering nucleosome positioning to control DNA accessibility.
SS18 gained prominence due to its role in synovial sarcoma, a rare soft-tissue malignancy. In ~95% of cases, a chromosomal translocation t(X;18) fuses *SS18* with *SSX* genes (e.g., *SSX1/2/4*), producing an oncogenic SS18-SSX fusion protein. This aberrant protein disrupts BAF complex composition and function, displacing wild-type SS18 and recruiting repressive Polycomb complexes. The resultant dysregulation of transcriptional programs drives tumorigenesis by impairing differentiation and promoting proliferation.
Beyond cancer, SS18 is involved in developmental processes and homeostasis. Recombinant SS18 protein is utilized in studies dissecting chromatin remodeling mechanisms, oncogenic fusion effects, and potential therapeutic targeting. Its interplay with epigenetic regulators underscores its broader relevance in diseases linked to transcriptional dysregulation. Research continues to explore SS18's physiological roles and its potential as a biomarker or therapeutic target in synovial sarcoma and other pathologies.
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