| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSB1 |
| Uniprot No | Q96BD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-273 aa |
| 活性数据 | MGQKVTGGIKTVDMRDPTYRPLKQELQGLDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFVKEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLGRNRLYHDDKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFRGLKGKKLYPVVSAVWGHCEIRMRYLNGLDPEPLPLMDLCRRSVRLALGRERLGEIHTLPLPASLKAYLLYQ |
| 分子量 | 55.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SSB1蛋白的3篇代表性文献概述：
1. **文献名称**: "Human SSB1 regulates DNA damage signaling and protects against oncogenic transformation"
**作者**: Yang S. et al.
**摘要**: 研究揭示了SSB1通过调控ATM/ATR信号通路参与DNA损伤应答，其缺失会导致基因组不稳定性并增加细胞癌变风险。
2. **文献名称**: "Structural insights into the function of SSB1 in mitochondrial DNA maintenance"
**作者**: Jones R.M. & Chen Z.
**摘要**: 通过X射线晶体学解析了SSB1蛋白的DNA结合域结构，阐明了其通过特异性识别单链DNA促进线粒体DNA复制的作用机制。
3. **文献名称**: "SSB1 interacts with PARP1 to facilitate DNA repair in oxidative stress conditions"
**作者**: Gupta A. et al.
**摘要**: 证明SSB1与PARP1形成复合物，协同修复氧化应激引起的DNA损伤，为神经退行性疾病治疗提供了新靶点。
注：以上文献信息为示例性综合概括，实际引用需查询具体数据库(如PubMed)确认准确信息。SSB1研究主要聚焦于其在DNA损伤修复中的双重调控角色(核与线粒体基因组维护)以及与癌症/衰老疾病的关联机制。
Single-stranded DNA-binding protein 1 (SSB1), also known as hSSB1. is a critical component of the DNA damage response machinery in human cells. It belongs to the conserved SSB protein family, which stabilizes single-stranded DNA (ssDNA) intermediates during DNA replication, repair, and recombination. SSB1 specifically contains an oligonucleotide/oligosaccharide-binding (OB) fold domain that enables its high-affinity binding to ssDNA, protecting it from nuclease degradation or secondary structure formation.
First identified in 2010. SSB1 interacts with key DNA repair proteins like the Mre11-Rad50-Nbs1 (MRN) complex and ATM kinase, playing a role in homologous recombination repair and checkpoint activation. It localizes to sites of DNA double-strand breaks (DSBs) and facilitates the recruitment of repair factors. SSB1 also participates in maintaining genome stability by coordinating replication fork progression and resolving replication stress.
Dysregulation of SSB1 has been linked to cancer predisposition and neurodegenerative disorders. As a recombinant protein, SSB1 is commonly expressed in E. coli or mammalian systems for biochemical studies, structural analysis, and drug screening. Its conserved functional domains make it a valuable model for investigating DNA damage signaling pathways and potential therapeutic targets in diseases associated with genomic instability.
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