| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST7 |
| Uniprot No | Q9NRC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-547 aa |
| 活性数据 | MAEAATGFLEQLKSCIVWSWTYLWTVWFFIVLFLVYILRVPLKINDNLSTVSMFLNTLTPKFYVALTGTSSLISGLILIFEWWYFRKYGTSFIEQVSVSHLRPLLGGVDNNSSNNSDSNRQSVSECKVWRNPLNLFRGAEYNRYTWVTGREPLTYYDMNLSAQDHQTFFTCDSDHLRPADAIMQKAWRERNPQARISAAHEALEINECATAYILLAEEEATTIAEAEKLFKQALKAGDGCYRRSQQLQHHGSQYEAQHRRDTNVLVYIKRRLAMCARRLGRTREAVKMMRDLMKEFPLLSMFNIHENLLEALLELQAYADVQAVLAKYDDISLPKSATICYTAALLKARAVSDKFSPEAASRRGLSTAEMNAVEAIHRAVEFNPHVPKYLLEMKSLILPPEHILKRGDSEAIAYAFFHLAHWKRVEGALNLLHCTWEGTFRMIPYPLEKGHLFYPYPICTETADRELLPSFHEVSVYPKKELPFFILFTAGLCSFTAMLALLTHQFPELMGVFAKAFLSTLFAPLNFVMEKVESILPSSLWHQLTRI |
| 分子量 | 89.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ST7蛋白的虚构示例参考文献(由于ST7蛋白相关研究较少，建议通过PubMed或Google Scholar检索真实文献)：
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1. **文献名称**: *ST7 suppresses tumor metastasis by regulating epithelial-mesenchymal transition in breast cancer cells*
**作者**: Zhang L, et al.
**摘要**: 研究利用重组人ST7蛋白在体外模型中验证其抑制乳腺癌细胞迁移和侵袭的能力，表明ST7通过调控E-cadherin表达抑制EMT过程。
2. **文献名称**: *Expression and purification of recombinant human ST7 protein for functional studies*
**作者**: Wang Y, et al.
**摘要**: 描述了在大肠杆菌中高效表达重组ST7蛋白的方法，并通过体外实验证实其与细胞表面整合素的相互作用，为后续机制研究提供工具。
3. **文献名称**: *The role of ST7 in Wnt/β-catenin signaling and colorectal cancer progression*
**作者**: Kim J, et al.
**摘要**: 发现重组ST7蛋白可拮抗Wnt信号通路，抑制结直肠癌细胞增殖，提示其作为潜在肿瘤抑制因子的功能。
4. **文献名称**: *Structural characterization of the ST7 protein and its interaction with lipid membranes*
**作者**: Müller S, et al.
**摘要**: 通过X射线晶体学解析ST7蛋白结构，揭示其疏水结构域在细胞膜结合中的作用，为理解其生物学功能提供结构基础。
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**注意**：以上为模拟示例，实际文献请通过学术数据库检索。ST7蛋白的研究可能涉及肿瘤抑制、信号通路调控或细胞黏附机制，建议结合具体研究目标筛选文献。
**Background of Recombinant Human ST7 Protein**
The recombinant human ST7 protein is a engineered form of the Suppression of Tumorigenicity 7 (ST7) protein, encoded by the *ST7* gene located on chromosome 7q31.2. ST7 is implicated in tumor suppression, with studies linking its loss or mutation to cancers such as breast, ovarian, and gastric malignancies. It is associated with regulating cell adhesion, proliferation, and signaling pathways, notably through interactions with the Wnt/β-catenin and integrin-mediated pathways.
Structurally, the recombinant ST7 protein is typically produced using expression systems like *E. coli* or mammalian cells (e.g., HEK293), enabling post-translational modifications crucial for functional studies. It often includes tags (e.g., His-tag) for purification and detection. Research highlights its role in inhibiting tumor cell invasion and metastasis by modulating extracellular matrix interactions and downregulating oncogenic signals.
Beyond oncology, ST7 is studied in neurological contexts, as it may influence neuronal migration and brain development. Its recombinant form serves as a tool to dissect molecular mechanisms in disease models, screen therapeutic agents, or develop diagnostics. Despite progress, its full mechanistic landscape and therapeutic potential remain under exploration, emphasizing the need for further structural and functional characterization.
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