| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUPV3L1 |
| Uniprot No | Q8IYB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-786 aa |
| 活性数据 | AICSALRP HFGPFPGVLG QVSVLATASS SASGGSKIPN TSLFVPLTVK PQGPSADGDV GAELTRPLDK NEVKKVLDKF YKRKEIQKLG ADYGLDARLF HQAFISFRNY IMQSHSLDVD IHIVLNDICF GAAHADDLFP FFLRHAKQIF PVLDCKDDLR KISDLRIPPN WYPDARAMQR KIIFHSGPTN SGKTYHAIQK YFSAKSGVYC GPLKLLAHEI FEKSNAAGVP CDLVTGEERV TVQPNGKQAS HVSCTVEMCS VTTPYEVAVI DEIQMIRDPA RGWAWTRALL GLCAEEVHLC GEPAAIDLVM ELMYTTGEEV EVRDYKRLTP ISVLDHALES LDNLRPGDCI VCFSKNDIYS VSRQIEIRGL ESAVIYGSLP PGTKLAQAKK FNDPNDPCKI LVATDAIGMG LNLSIRRIIF YSLIKPSINE KGERELEPIT TSQALQIAGR AGRFSSRFKE GEVTTMNHED LSLLKEILKR PVDPIRAAGL HPTAEQIEMF AYHLPDATLS NLIDIFVDFS QVDGQYFVCN MDDFKFSAEL IQHIPLSLRV RYVFCTAPIN KKQPFVCSSL LQFARQYSRN EPLTFAWLRR YIKWPLLPPK NIKDLMDLEA VHDVLDLYLW LSYRFMDMFP DASLIRDLQK ELDGIIQDGV HNITKLIKMS ETHKLLNLEG FPSGSQSRLS GTLKSQARRT RGTKALGSKA TEPPSPDAGE LSLASRLVQQ GLLTPDMLKQ LEKEWMTQQT EHNKEKTESG THPKGTRRKK KEPDSD |
| 分子量 | 87.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"SUPV3L1 regulates the mitochondrial unfolded protein response and promotes cancer progression" by Li et al.**
摘要：研究揭示了重组SUPV3L1蛋白在线粒体未折叠蛋白反应(UPRmt)中的调控作用，通过其解旋酶活性影响线粒体应激信号传导，并促进肿瘤细胞的存活与转移。
2. **"Biochemical characterization of the human SUPV3L1 helicase: ATP hydrolysis and nucleic acid binding properties" by Radisky et al.**
摘要：该文献报道了重组人SUPV3L1蛋白的体外表达与纯化，并分析其ATP酶活性和对DNA/RNA底物的结合能力，证实其在核酸代谢中的核心功能。
3. **"SUPV3L1 deficiency disrupts mitochondrial RNA processing and exacerbates oxidative stress" by Wang et al.**
摘要：通过构建重组SUPV3L1突变体，研究其在线粒体RNA加工中的作用，表明该蛋白缺失会导致RNA代谢异常及氧化应激水平升高。
4. **"SUPV3L1 interacts with mitochondrial polymerase POLG to maintain genome stability" by Cheng et al.**
摘要：利用重组蛋白共表达实验，证明SUPV3L1与线粒体DNA聚合酶POLG相互作用，协同维持线粒体基因组稳定性，并影响细胞衰老进程。
(注：以上文献信息为模拟生成，实际文献需通过学术数据库检索确认。)
The SUPV3L1 protein, also known as human Suv3 helicase or RNA helicase SUV3-like protein 1. is a conserved mitochondrial enzyme belonging to the DExH-box helicase family. Encoded by the *SUPV3L1* gene, it plays a critical role in mitochondrial RNA metabolism, including RNA processing, degradation, and quality control. This ATP-dependent helicase is essential for maintaining mitochondrial genome stability by resolving RNA-DNA hybrids and eliminating aberrant transcripts. Studies link SUPV3L1 to mitochondrial DNA replication, stress response, and apoptosis regulation. Dysregulation of SUPV3L1 has been implicated in mitochondrial disorders, neurodegenerative diseases, and cancer progression. Recombinant human SUPV3L1 protein, typically produced in *E. coli* or mammalian expression systems, enables biochemical studies of its helicase activity and RNA/DNA interaction mechanisms. Its purified form is widely used to investigate mitochondrial gene expression pathways, antiviral defense systems, and connections between mitochondrial dysfunction and age-related pathologies. Recent research also explores its potential as a therapeutic target for metabolic syndromes and its interplay with nuclear DNA repair processes.
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