| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUHW4 |
| Uniprot No | Q6N043 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158 aa |
| 活性数据 | MGDNPFQPKSNSKMAELFMECEEEELEPWQKKVKEVEDDDDDEPIFVGEISSSKPAISNILNRVNPSSYSRGLKNGALSRGITAAFKPTSQHYTNPTSNPVPASPINFHPESRSSDSSVIVQPFSKPVSVSKTIRPAQGSIGCCLSISTVPSYNSGLS |
| 分子量 | 43.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设生成的模拟参考文献示例。由于“SUHW4”可能并非标准命名，建议核实名称准确性(如是否可能为“SUMO4”、“SAW4”或其他变体)：
1. **文献名称**: *Cloning and Functional Analysis of Human SUHW4 in Chromatin Insulator Activity*
**作者**: Zhang L. et al.
**摘要**: 本研究克隆了人SUHW4基因并在HEK293细胞中表达重组蛋白，发现其通过结合CTCF协同调控染色质绝缘子功能，影响基因组三维结构。
2. **文献名称**: *SUHW4 Interacts with Cohesin Complex to Maintain Genomic Stability*
**作者**: Kim S., Patel R.
**摘要**: 通过免疫共沉淀证实SUHW4与黏连蛋白复合体相互作用，敲低SUHW4导致染色体分离异常，提示其在有丝分裂中起关键作用。
3. **文献名称**: *Structural Characterization of Recombinant SUHW4 Zinc Finger Domain*
**作者**: Müller F. et al.
**摘要**: 利用X射线晶体学解析了SUHW4蛋白锌指结构域的三维结构，揭示了其与DNA结合的分子机制，为突变导致神经发育障碍提供结构基础。
4. **文献名称**: *SUHW4 Epigenetic Dysregulation in Breast Cancer Metastasis*
**作者**: Garcia M. et al.
**摘要**: 发现乳腺癌中SUHW4表达下调与患者预后不良相关，体外实验表明其通过抑制EMT通路抑制肿瘤转移。
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**重要提示**：
- SUHW4可能为虚构或非标准命名，建议确认名称正确性(例如果蝇Su(Hw)的人类同源蛋白或转录因子相关名称)。
- 上述文献为模拟示例，实际研究中请通过PubMed或Google Scholar使用准确关键词检索。
**Background of Recombinant Human SUHW4 Protein**
SUHW4 (Suppressor of Hairy-wing Homolog 4), also known as ARIP4 or RAD54L2. is a member of the SNF2-like family of chromatin remodeling proteins. It plays a role in transcriptional regulation, DNA repair, and chromatin dynamics by leveraging its ATP-dependent nucleosome remodeling activity. SUHW4 contains a helicase domain critical for ATP hydrolysis and a C-terminal domain implicated in protein interactions, potentially linking chromatin remodeling to transcriptional machinery or DNA damage response pathways.
Biologically, SUHW4 is involved in androgen receptor (AR)-mediated signaling, suggesting a role in hormone-dependent processes, including prostate cancer progression. Studies also associate it with genome stability, as it participates in homologous recombination repair, highlighting its dual function in gene expression regulation and DNA repair mechanisms.
Recombinant human SUHW4 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling study of its biochemical properties, interactome, and functional roles *in vitro*. Its purified form facilitates research on chromatin remodeling mechanisms, cancer biology, and therapeutic targeting of AR-dependent diseases. Current investigations focus on elucidating its precise molecular interactions and potential as a diagnostic or therapeutic biomarker in oncology and genetic disorders.
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