| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUHW1 |
| Uniprot No | P59817 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542 aa |
| 活性数据 | MGDIFLCKKV ESPKKNLRES KQREEDDEDP DLIYVGVEHV HRDAEVLFVG MISNSKPVVS NILNRVTPGS KSRRKKGHFR QYPAHVSQPA NHVTSMAKAI MPVSLSEGRS TDSPVTMKSS SEPGYKMSSP QVVSPNYSDS LPPGTQCLVG AMVSGGGRNE SSPDSKRLST SDINSRDSKR VKLRDGIPGV PSLAVVPSDM SSTISTNTPS QGICNSSNHV QNGVTFPWPD ANGKAHFNLT DPERANESGL AMTDISSLAS QNKTFDPKKE NPIVLLSNFY YGQHKGDGQP EQKTHTTFKC LSCVKVLKNI KFMNHMKHHL EFEKQRNDSW EDHTTCQHCH RQFPTPFQLQ CHIDSVHIAM GPSAVCKICE LSFETDQVLL QHMKDHHKPG EMPYVCQVCH YRSSVFADVE THFRTCHENT KNLLCLFCLK LFKTAIPYMN HCWRHSRRRV LQCSKCRLQF LTLKEEIEHK TKDHQTFKKP EQLQGFPRET KVIIQTSVQP GSSGMASVIV SNTDPQSSPV KTKKKTAMNT RDSRLPCSKD SS |
| 分子量 | 60.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人SUHW1蛋白相关研究的文献摘要(文献名称与内容为模拟示例，仅作格式参考)：
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1. **文献名称**：SUHW1 mediates transcriptional repression through interaction with chromatin insulator complexes
**作者**：Johnson et al. (2019)
**摘要**：研究揭示SUHW1作为染色质绝缘子结合蛋白，通过重组实验证实其与CTCF协同作用，抑制基因座间的非特异性增强子-启动子互作，维持三维基因组稳定性。
2. **文献名称**：Structural analysis of recombinant SUHW1 zinc-finger domains in boundary element formation
**作者**：Lee & Zhang (2021)
**摘要**：通过重组SUHW1蛋白的锌指结构域晶体学研究，阐明其靶向DNA特定基序的分子机制，为染色质区室化调控提供结构基础。
3. **文献名称**：SUHW1 knockout disrupts Notch signaling and leads to embryonic lethality in mice
**作者**：Wang et al. (2016)
**摘要**：利用重组SUHW1蛋白进行体外挽救实验，证明其在Notch通路中通过抑制Suppressor of Hairless(RBPJ)复合物活性调控神经发育进程。
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**注**：以上内容为模拟文献概括，实际文献需通过PubMed/Google Scholar等平台以“SUHW1”“Recombinant SUHW1”“chromatin insulator”等关键词检索获取。
**Background of Recombinant Human SUHW1 Protein**
SUHW1 (also known as Zinc Finger Protein 280D, ZNF280D) is a chromatin-associated protein that functions as a transcriptional repressor. It belongs to the zinc finger protein family, characterized by C2H2-type zinc finger domains, which mediate sequence-specific DNA binding and protein-protein interactions. SUHW1 is best known for its role in the ATRX/DNMT3-DNMT3L (ADDN) complex, where it assists in targeting the complex to specific genomic regions, such as pericentric heterochromatin and telomeres. This complex plays a critical role in maintaining genome stability through chromatin remodeling, DNA methylation, and gene silencing.
SUHW1 is involved in epigenetic regulation, particularly in silencing repetitive elements and regulating imprinted genes. Dysregulation of SUHW1 has been linked to developmental disorders and cancer, where its aberrant expression may contribute to genomic instability or aberrant gene expression. Recombinant human SUHW1 protein is typically produced using bacterial or eukaryotic expression systems, enabling studies on its structure, interactions, and biochemical functions. Researchers utilize this protein to explore mechanisms of chromatin organization, transcriptional repression, and its potential as a therapeutic target. Its recombinant form is essential for *in vitro* assays, structural studies, and disease modeling.
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