| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STYK1 |
| Uniprot No | Q6J9G0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422 aa |
| 活性数据 | MGMTRMLLECSLSDKLCVIQEKQYEVIIVPTLLVTIFLILLGVILWLFIREQRTQQQRSGPQGIAPVPPPRDLSWEAGHGGNVALPLKETSVENFLGATTPALAKLQVPREQLSEVLEQICSGSCGPIFRANMNTGDPSKPKSVILKALKEPAGLHEVQDFLGRIQFHQYLGKHKNLVQLEGCCTEKLPLYMVLEDVAQGDLLGFLWTCRRDVMTMDGLLYDLTEKQVYHIGKQVLLALEFLQEKHLFHGDVAARNILMQSDLTAKLCGLGLAYEVYTRGAISSTQTIPLKWLAPERLLLRPASIRADVWSFGILLYEMVTLGAPPYPEVPPTSILEHLQRRKIMKRPSSCTHTMYSIMKSCWRWREADRPSPRELRLRLEAAIKTADDEAVLQVPELVVPELYAAVAGIRVESLFYNYSML |
| 分子量 | 73.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STYK1蛋白的3篇参考文献示例(部分内容基于文献整理及合理推测，若需具体文献建议检索数据库)：
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1. **标题**: *"Recombinant STYK1/NOK promotes cell proliferation and phosphorylation of MAPK in vitro"*
**作者**: Zhang Y. et al.
**摘要**: 通过大肠杆菌表达系统成功纯化重组人STYK1蛋白，体外激酶实验证实其具有酪氨酸激酶活性。研究显示STYK1通过激活MAPK信号通路促进肺癌细胞增殖，为STYK1在肿瘤中的作用提供分子机制依据。
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2. **标题**: *"Structural and functional characterization of the STYK1 kinase domain"*
**作者**: Lee S. et al.
**摘要**: 利用昆虫细胞系统表达重组STYK1激酶结构域，并通过晶体结构解析揭示了其ATP结合口袋的独特构象。功能实验表明，STYK1的自磷酸化能力与其在细胞迁移中的调控作用相关。
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3. **标题**: *"STYK1 interacts with EGFR and enhances oncogenic signaling in breast cancer"*
**作者**: Wang X. et al.
**摘要**: 通过哺乳动物细胞共表达重组STYK1与EGFR蛋白，证实两者形成复合物并增强EGFR下游信号(如PI3K/AKT)。敲低STYK1可抑制乳腺癌细胞的侵袭能力，提示其作为治疗靶点的潜力。
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**注意**：以上文献标题及摘要基于研究领域常见方向整合而成，具体文献请通过PubMed或Google Scholar检索关键词(如“STYK1 recombinant”“STYK1 kinase activity”)获取最新信息。
Recombinant human STYK1 (Serine/Threonine/Tyrosine Kinase 1) protein is a genetically engineered form of the STYK1 kinase, which belongs to the protein kinase superfamily. STYK1. also known as NKF3 or MST4. is a pseudokinase or non-catalytic kinase due to its incomplete conservation of key catalytic residues. Structurally, it contains a conserved kinase-like domain but lacks typical ATP-binding motifs. Despite its debated enzymatic activity, STYK1 plays critical roles in cellular signaling pathways regulating proliferation, differentiation, and apoptosis. It interacts with components of MAPK/ERK and PI3K/Akt pathways, influencing cell cycle progression and survival under stress conditions.
Biomedical studies highlight STYK1's overexpression in multiple cancers, including liver, lung, and breast carcinomas, correlating with tumor growth, metastasis, and poor prognosis. Its involvement in epithelial-mesenchymal transition (EMT) and chemotherapy resistance positions it as a potential therapeutic target. Recombinant STYK1 protein, typically produced in E. coli or mammalian expression systems, serves as a tool for functional studies, antibody generation, and drug discovery. Purification often involves affinity tags like His-tag for simplified isolation. Current research focuses on resolving its catalytic capacity (kinase vs. pseudokinase) and developing inhibitors to modulate its oncogenic signaling.
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