| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX18 |
| Uniprot No | Q9P2W9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335 aa |
| 活性数据 | MAVDITLLFRASVKTVKTRNKALGVAVGGGVDGSRDELFRRSPRPKGDFSSRAREVISHIGKLRDFLLEHRKDYINAYSHTMSEYGRMTDTERDQIDQDAQIFMRTCSEAIQQLRTEAHKEIHSQQVKEHRTAVLDFIEDYLKRVCKLYSEQRAIRVKRVVDKKRLSKLEPEPNTKTRESTSSEKVSQSPSKDSEENPATEERPEKILAETQPELGTWGDGKGEDELSPEEIQMFEQENQRLIGEMNSLFDEVRQIEGRVVEISRLQEIFTEKVLQQEAEIDSIHQLVVGATENIKEGNEDIREAIKNNAGFRVWILFFLVMCSFSLLFLDWYDS |
| 分子量 | 62.48 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人STX18蛋白相关的文献参考示例(部分内容为模拟构建，供参考)：
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1. **文献名称**: *"Functional characterization of recombinant human STX18 in ER-associated membrane fusion"*
**作者**: Tanaka K, et al.
**摘要**: 本研究利用大肠杆菌重组表达系统制备了人源STX18蛋白，揭示了其在内质网膜融合中的核心作用。通过体外膜重组实验，证明STX18与Sec22b等SNARE蛋白协同介导内质网-高尔基体间的囊泡运输，并验证了其N端结构域对功能的关键影响。
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2. **文献名称**: *"Structural insights into the STX18-SNARE complex in autophagy regulation"*
**作者**: Wang L, et al.
**摘要**: 通过X射线晶体学解析重组人STX18蛋白的C端结构域，发现其与SNAP29和VAMP8形成复合物的关键相互作用位点，揭示了STX18通过调控自噬体-溶酶体融合参与细胞应激反应的分子机制。
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3. **文献名称**: *"STX18 overexpression promotes colorectal cancer metastasis via exosome secretion modulation"*
**作者**: Chen Y, et al.
**摘要**: 利用重组人STX18蛋白进行体外功能研究，发现其过表达通过增强多泡体与质膜融合效率，促进结直肠癌细胞外泌体释放，激活TGF-β信号通路并驱动肿瘤转移。
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4. **文献名称**: *"Interaction mapping of STX18 with COPII components using recombinant protein pulldown assays"*
**作者**: Liu X, et al.
**摘要**: 通过重组STX18蛋白的pull-down实验结合质谱分析，鉴定了其与COPII囊泡运输相关蛋白(如Sec23/Sec24)的直接互作，表明STX18可能通过协调COPII复合体功能参与分泌途径的早期阶段调控。
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**注意**：以上文献信息为示例性构建，实际研究中请通过PubMed或Web of Science等平台检索真实发表文献。若需具体文献，可补充关键词(如疾病模型或实验方法)进一步筛选。
**Recombinant human STX18 protein** is a genetically engineered form of the Syntaxin 18 (STX18) protein, a member of the syntaxin family involved in intracellular membrane trafficking. STX18 localizes primarily to the endoplasmic reticulum (ER) and plays a critical role in regulating vesicle transport between the ER and Golgi apparatus. It mediates membrane fusion events by interacting with other SNARE (soluble NSF attachment protein receptor) proteins, such as Sec22b and BNIP1. facilitating cargo sorting and organelle communication. Structurally, STX18 contains a characteristic SNARE domain, a transmembrane region, and coiled-coil motifs that enable protein-protein interactions.
Research on recombinant STX18 has surged due to its implications in cellular homeostasis, secretion pathways, and disease mechanisms. Dysregulation of STX18 is linked to cancers, neurodegenerative disorders, and metabolic conditions, highlighting its therapeutic potential. Recombinant versions are produced using bacterial or mammalian expression systems, enabling studies on its biochemical properties, interaction networks, and role in ER stress responses. Additionally, it serves as a tool for developing targeted therapies or diagnostics. Understanding STX18’s molecular functions advances insights into vesicle trafficking dysfunctions and their broader physiological impacts.
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